Cell surface plates or flasks

The well-characterized human bronchial epithelial cell lines, BEAS2-B (WT), CuFi-1(ΔF508/ΔF508), CuFi-4 (G551D/ΔF508) were obtained from ATCC, all the details regarding these cell lines are deposited in their web page (see table of reagents). All cells were grown on Cell+ surface plates or flasks (Sarstedt). BEAS-2B were cultured in LHC basal medium (ThermoFisher Scientific) supplemented with 10% FBS, 50 U/ml penicillin and 50 μg/ml streptomycin). CuFi-1 and CuFi-4 were cultured in LHC-9 medium (ThermoFisher Scientific), as described by the manufacturer.

Human cell lines BEAS-2B (ATCC CRL-9609), CuFi-1 (ATCC CRL-4013), CuFi-4 (ATCC CRL-4015) and IB3-1 (ATCC CRL-2777) were purchase from ATCC (UK) which ensures STR profiling of the cell lines used. BEAS-2B and IB3-1 were cultured in LHC basal medium (Thermo Fisher Scientific, Loughborough, UK) supplemented with 10% FBS, 50 U/ml penicillin and 50 μg/ml streptomycin). CuFi-1 and CuFi-4 were grown on Cell+ surface plates or flasks (Sarstedt, Leicester, UK) with LHC-9 medium (Thermo Fisher Scientific, Loughborough, UK). All cells were cultured in a humidified incubator at 37°C, 5% CO₂. Cells were used at 1 × 10⁶/ ml. Cell lines were routinely tested for mycoplasma using MycoAlertTM Mycoplasma Detection Kit Lonza catalog#: LT07-118, and were all negative.