α-Synuclein for infrared studies (280 μM) was prepared in 20 mM HEPES, 150 mM NaCl, pD 7.40. Then, 400 μM of CMT-3, also in the same buffer, was added and the mixture incubated for 16 h under orbital agitation at 37 °C. To avoid αSm contribution, an Amicon Ultra-0.5 100 kDa cut-off filter was used recovering about 50 μl from the upper side of the filtration system. Each sample was assembled in a liquid cell (Harricks Scientific, Ossioning, NY) between two CaF2 windows with a path length of 50 nm. The sample chamber was constantly purged with dry air. The spectra were obtained by averaging 256 interferograms collected with a nominal resolution of 2 cm−1 and apodized with a Happ-Genzel function in a Nicolet 5700 spectrometer equipped with a DTGS detector (Thermo Nicolet, Madison, WI) as previously described74 (link). Quantitative information on protein structure was obtained through decomposition of the Amide I' band into its constituents as previously described75 (link),76 (link). Structural analyses, either in the absence or in the presence of CMT-3 were repeated three times to test the reproducibility of the measurements. In each case, the differences among the experiments was around 3%77 (link).
Nicolet 5700 spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Nicolet 5700 spectrometer is a Fourier-transform infrared (FTIR) spectroscopy instrument designed for analytical applications. It provides high-resolution infrared spectral data for identifying and characterizing organic and inorganic materials. The Nicolet 5700 spectrometer is capable of analyzing solid, liquid, and gas samples.
Automatically generated - may contain errors
Lab products found in correlation
Escalab 250xi, by Thermo Fisher Scientific (4 mentions)
Agilent 6520 hplc q tof, by Agilent Technologies (3 mentions)
Diaion hp 20, by Mitsubishi (3 mentions)
Lc 10at, by Shimadzu (3 mentions)
Dtgs detector, by Thermo Fisher Scientific (2 mentions)
Agilent 1200 series system, by Agilent Technologies (2 mentions)
600 mhz nmr spectrometer, by Agilent Technologies (2 mentions)
P 2000, by Jasco (2 mentions)
V 650, by Jasco (2 mentions)
P 2000 polarimeter, by Jasco (2 mentions)
V 650 spectrophotometer, by Jasco (2 mentions)
Cary 50 spectrophotometer, by Agilent Technologies (1 mentions)
Ultima 4 multipurpose xrd system, by Rigaku (1 mentions)
Avance 300 spectrometer, by Bruker (1 mentions)
X pert diffractometer, by Malvern Panalytical (1 mentions)
Avance 3 800, by Bruker (1 mentions)
Spd 20a detector, by Shimadzu (1 mentions)
Lc 10a, by Shimadzu (1 mentions)
J 815 cd spectrometer, by Jasco (1 mentions)
Sp 700, by Mitsubishi (1 mentions)
47 protocols using nicolet 5700 spectrometer
1
Infrared Structural Analysis of α-Synuclein
2
Spectroscopic Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents were purchased from
Honeywell, Alfa Aesar, and Sigma-Aldrich and used without further
purification. Solvents (reagent grade) were purchased from Honeywell,
VWR, and Merck and dried by using standard techniques when required.
Manipulations were performed using standard Schlenk techniques under
a dry dinitrogen atmosphere. Elemental analyses were performed with
a CHNS/O PE 2400 series II CHNS/O elemental analyzer (T = 925 °C). FT-IR spectra were recorded with a Thermo-Nicolet
5700 spectrometer at room temperature: KBr pellets with a KBr beam
splitter and KBr windows (4000–400 cm–1,
resolution 4 cm–1) were used. Absorption spectra
were recorded at 25 °C in a quartz cell of 10.00 mm optical path
with either a Thermo Evolution 300 (190–1100 nm) spectrophotometer
or an Agilent Cary 5000 UV–vis–NIR (190–2000
nm) dual-beam spectrophotometer. Absorption spectra were decomposed
into their constituent Gaussian peaks using the Specpeak 2.065 (link) and Fityk 1.3.166 (link) programs. The Crystallographic Structural Database was accessed
by using CCDC ConQuest 2020.1.67 (link)
Honeywell, Alfa Aesar, and Sigma-Aldrich and used without further
purification. Solvents (reagent grade) were purchased from Honeywell,
VWR, and Merck and dried by using standard techniques when required.
Manipulations were performed using standard Schlenk techniques under
a dry dinitrogen atmosphere. Elemental analyses were performed with
a CHNS/O PE 2400 series II CHNS/O elemental analyzer (T = 925 °C). FT-IR spectra were recorded with a Thermo-Nicolet
5700 spectrometer at room temperature: KBr pellets with a KBr beam
splitter and KBr windows (4000–400 cm–1,
resolution 4 cm–1) were used. Absorption spectra
were recorded at 25 °C in a quartz cell of 10.00 mm optical path
with either a Thermo Evolution 300 (190–1100 nm) spectrophotometer
or an Agilent Cary 5000 UV–vis–NIR (190–2000
nm) dual-beam spectrophotometer. Absorption spectra were decomposed
into their constituent Gaussian peaks using the Specpeak 2.065 (link) and Fityk 1.3.166 (link) programs. The Crystallographic Structural Database was accessed
by using CCDC ConQuest 2020.1.67 (link)
3
FTIR Analysis of α-Synuclein Aggregation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples at 280 μM of α-synuclein in the absence or the presence of 400 μM doxycycline in buffer 20 mM HEPES, 150 mM NaCl, pD 7.0, D2O were collected after 0, 2 and 16 h of orbital incubation at 37 °C. Since monomeric α-synuclein was higher than the oligomeric species, after incubation we partially separated from the monomer using an Amicon Ultra-0.5 100 kDa cut-off filter and assembled in a thermostated cell between two CaF2 windows with a path length of 50 nm. The spectra were recorded in a Nicolet 5700 spectrometer equipped with a DTGS detector (Thermo Nicolet, Madison, WI) as previously described29 (link). The sample chamber was permanently purged with dry air. The spectra were generated by averaging 256 interferograms collected with a nominal resolution of 2 cm−1 and apodized with a Happ-Genzel function. The D2O contribution in the amide I´ region was eliminated by subtracting the buffer spectra from that of the solution at the same temperature to obtain a flat baseline between 1,900 and 1,700 cm−1. Solvent subtraction, deconvolution, determination of band position and curve fitting of the original amide I band were performed as described29 (link). The error in determination of the FTIR structural analysis from the amide I band from different runs is 3%.
4
FTIR Spectroscopy Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fourier transform infrared spectroscopy (FTIR) spectra for different specimens were recorded on a Nicolet5700 spectrometer (Thermo Nicolet Corporation, Madison, WI, USA) with the wavenumber ranging from 750 to 4000 cm−1.
5
Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents were used as purchased from Aldrich or Fluka. Elemental analyses were performed using a Fisons Instruments 1108 CHNS elemental analyser. FT-infrared spectra of powdered samples were recorded with a Thermo-Nicolet 5700 spectrometer from 4000 to 400 cm -1 in the form of pressed KBr pellets. UV-vis spectrophotometric measurements were carried out with a Varian Cary 50 spectrophotometer equipped with a fiber optic dip probe (1 cm optical path length).
6
Characterization of Synthesized Dense BGN and MBGN
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The synthesized dense BGN and MBGN were characterized as follows. The crystalline phase was determined via x-ray diffraction (XRD) analysis using 40 kV, 40 mA Cu-Kɑ radiation, and an X-ray diffractometer (Ultima IV multipurpose XRD system, Rigaku, The Woodland, TX, USA). XRD analysis was performed with a scanning speed of 4°/min and 2θ range of 10–70° for wide-angle XRD patterns, and with a scanning speed of 1.2°/min and range of 0.5–10° for small-angle XRD patterns. Functional groups and chemical composition were examined via Fourier-transform infrared spectroscopy (FTIR) analyses using a Nicolet 5700 spectrometer (Thermo Scientific Inc., Madison, WI, USA). Information about N2 adsorption-desorption isotherms was obtained at 77 K using an ASAP 2420 gas adsirotuib analyzer (Micromeritics, Atlanta, GA, USA). Specific surface area and pore size distribution were calculated via the BET and BJH method, respectively. The topography of the sample was observed and analyzed with field emission scanning electron microscopy (FESEM) (Sigma, Zeiss, Germany).
7
Comprehensive Characterization of LDH Precursors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Powder X-ray diffraction (PXRD) data were obtained using a Panalytical X’pert diffractometer with a CuKα radiation (λ = 1.54155 Å). Fourier-transform infrared spectroscopy (FTIR) measurements were carried out on solid products using a Thermo Nicolet 5700 spectrometer, employing KBr tablets at a mass ratio of 1:100 (sample:KBr) with a resolution of 4 cm−1 and accumulation of 64 scans. The solid-state 27Al, 31P and 29Si NMR spectra were acquired using a Bruker AVANCE 300 spectrometer operating at 7.05 Tesla, equipped with a 4 mm zirconia multinuclear solids probe and magic angle spinning at 12 kHz. Thermagravimetric analysis (TGA) curves and differential thermal analysis (DTA), used to calculate the water content in the LDH precursors, were obtained with a TG-DTA SETSYS Evolution analyzer from SETARAM, using 150 μL alumina crucibles and a heating rate of 5 °C min−1 under air flow of 50 mL min−1.
The scanning electron microscopic (SEM) images were obtained using a Cambridge Scan 360 SEM operating at 1 kV and a Zeiss supra 55 FEG-VP operating at 3 keV. The samples were mounted on conductive carbon adhesive tabs for imaging.
The scanning electron microscopic (SEM) images were obtained using a Cambridge Scan 360 SEM operating at 1 kV and a Zeiss supra 55 FEG-VP operating at 3 keV. The samples were mounted on conductive carbon adhesive tabs for imaging.
8
Structural Analysis of Heparin Samples
9
Comprehensive Characterization of Chemical Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured on a Jasco P-2000 polarimeter (Jasco Corp; Tokyo, Japan). The UV spectra were measured on a Jasco V650 spectrophotometer (Jasco). The IR spectra were recorded on a Nicolet 5700 spectrometer (Thermo Scientific, FL). The CD spectra were measured on a Jasco J-815 CD spectrometer (Jasco). High-resolution electrospray ionization mass spectrometry (HR-ESI-MS) was performed on an Agilent 6520 HPLC-Q-TOF (Agilent Technologies, Waldbronn, Germany). 1H NMR (500 MHz), 13C NMR (125 MHz), and 2D NMR spectra were recorded with a Bruker 500 MHz spectrometer (Bruker-Biospin, Billerica, MA, USA) and values were given in ppm (δ). Column chromatography was carried out with macroporous resin (Diaion HP-20 and SP-700, Mitsubishi Chemical Corp, Tokyo, Japan) and Sephadex LH-20 (Pharmacia Fine Chemicals, Uppsala, Sweden). Flash chromatography was conducted using Combiflash RF200 (Teledyne Isco Corp, Nebraska, USA). Preparative HPLC was carried out on a Shimadzu LC-10A instrument with a SPD-20A detector (Shimadzu Corp, Tokyo, Japan), using YMC-Pack ODS-A column (250 mm×20 mm, 5 μm, YMC Corp, Kyoto, Japan). HPLC-DAD analysis was set up on Agilent 1200 series system (Agilent Technologies) with an Apollo C18 column (250 mm×4.6 mm, 5 μm, Alltech Corp, Kentucky, USA).
10
Comprehensive Characterization of Graphene-Based Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Raman spectrometer
used was a Horiba LabRAM HR Evolution system (λ = 633 nm) with
an 1800 lines/mm grating. The laser power was kept below 1 mW to avoid
damaging the samples. The polarization of the incident light was always
parallel to the deformation direction. A scanning electron microscope
(SEM, Tescan Mira 3 FEGSEM) was employed to characterize the morphology
of fibers coated with the graphene-based materials. All the specimens
were gold-coated before imaging. Fourier-transform infrared (FT-IR)
spectra were obtained in the transmission mode by using a Nicolet
5700 spectrometer (ThermoFisher Scientific Inc.). X-ray diffraction
patterns (XRD) were obtained using a PANalytical X’Pert X-ray
diffractometer (Philips) equipped with a Cu Kα radiation source
(λ = 1.542 Å). An X-ray photoelectron spectroscope (XPS)
equipped with a monoenergetic Al Kα X-ray source at 20 eV pass
energy with a step size of 100 meV was used. The morphology of the
graphene platelets was investigated using a NanoWizard atomic force
microscope (AFM) from JPK Instruments (Germany). Before imaging, a
small amount of graphene powder was dissolved in a mixture of isopropanol
and deionized water with a volume fraction of 1:1, which was then
sonicated for 2 h and drop-cast onto silicon wafers to be imaged.
used was a Horiba LabRAM HR Evolution system (λ = 633 nm) with
an 1800 lines/mm grating. The laser power was kept below 1 mW to avoid
damaging the samples. The polarization of the incident light was always
parallel to the deformation direction. A scanning electron microscope
(SEM, Tescan Mira 3 FEGSEM) was employed to characterize the morphology
of fibers coated with the graphene-based materials. All the specimens
were gold-coated before imaging. Fourier-transform infrared (FT-IR)
spectra were obtained in the transmission mode by using a Nicolet
5700 spectrometer (ThermoFisher Scientific Inc.). X-ray diffraction
patterns (XRD) were obtained using a PANalytical X’Pert X-ray
diffractometer (Philips) equipped with a Cu Kα radiation source
(λ = 1.542 Å). An X-ray photoelectron spectroscope (XPS)
equipped with a monoenergetic Al Kα X-ray source at 20 eV pass
energy with a step size of 100 meV was used. The morphology of the
graphene platelets was investigated using a NanoWizard atomic force
microscope (AFM) from JPK Instruments (Germany). Before imaging, a
small amount of graphene powder was dissolved in a mixture of isopropanol
and deionized water with a volume fraction of 1:1, which was then
sonicated for 2 h and drop-cast onto silicon wafers to be imaged.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!