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Erk p44 42 mapk erk1 2

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ERK: p44/42 MAPK (Erk1/2) is a laboratory product that functions as a protein kinase. It is involved in the regulation of various cellular processes, including cell proliferation, differentiation, and survival.

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Lab products found in correlation

Anti syk n 19, by Santa Cruz Biotechnology (2 mentions) H3 anti histone h3 pan, by Merck Group (2 mentions) Psyk anti phospho zap 70 tyr319 syk tyr352, by Cell Signaling Technology (2 mentions) Perk erk1 2 ptpy185 187, by Thermo Fisher Scientific (2 mentions) Ab152, by Merck Group (1 mentions) Islet 1 2, by Developmental Studies Hybridoma Bank (1 mentions) A1r multiphoton microscope, by Nikon (1 mentions) Ab125, by Merck Group (1 mentions)

Close spelling variants of this product

P44/42 MAPK (Erk1/2) ERK (p44/42 MAPK, ERK1/2 p44/42 P44/42 MAPK ERK p44/42 ERK1/2 MAPK ERK1/2

3 protocols using erk p44 42 mapk erk1 2

1

Neutrophil Signaling Pathway Activation

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1×106 human peripheral neutrophils were plated in 12-well plates. Neutrophils were stimulated with C. albicans yeast or pre-formed hyphae. Add indicated times cells were lysed in SDS sample buffer and stored at −80°C. A standard immunoblotting protocol was carried out as described elsewhere. H3: anti-histone H3 (pan) (Millipore), Syk: anti-Syk (N-19) (Santa Cruz), pSyk: anti-phospho-Zap-70 (Tyr319)/Syk (Tyr352) (Cell Signaling), ERK: p44/42 MAPK (Erk1/2) (Cell Signaling), pERK: ERK1/2 (pTpY185/187) (Life Technologies).
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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2

Larval Immunohistochemistry for Neuronal Markers

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Larval IHC was performed as previously published (Avants et al., 2011 (link)), using antibodies raised against total ERK (ERK; p44/42 MAPK [ERK1/2], #4696, Cell Signaling Inc, Danvers, MA, USA), Islet-1 and Islet-2 homeobox (Islet1/2, #39.4D5, Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA, USA), TH1 (AB152, Sigma-Aldrich Inc, Burlington, MA, USA), and 5-HT (AB125, Sigma-Aldrich Inc). IHC-stained larvae were imaged on a Nikon A1R multiphoton microscope, using a water immersion 25×, NA 1.1 objective.
Kozol R.A., Conith A.J., Yuiska A., Cree-Newman A., Tolentino B., Benesh K., Paz A., Lloyd E., Kowalko J.E., Keene A.C., Albertson C, & Duboue E.R. (2023). A brain-wide analysis maps structural evolution to distinct anatomical module. eLife, 12, e80777.
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3

Neutrophil Signaling Pathway Activation

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1×106 human peripheral neutrophils were plated in 12-well plates. Neutrophils were stimulated with C. albicans yeast or pre-formed hyphae. Add indicated times cells were lysed in SDS sample buffer and stored at −80°C. A standard immunoblotting protocol was carried out as described elsewhere. H3: anti-histone H3 (pan) (Millipore), Syk: anti-Syk (N-19) (Santa Cruz), pSyk: anti-phospho-Zap-70 (Tyr319)/Syk (Tyr352) (Cell Signaling), ERK: p44/42 MAPK (Erk1/2) (Cell Signaling), pERK: ERK1/2 (pTpY185/187) (Life Technologies).
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D, & Papayannopoulos V. (2014). Neutrophils sense microbial size and selectively release neutrophil extracellular traps in response to large pathogens. Nature immunology, 15(11), 1017-1025.
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