Imaging was performed with an inverted microscope (Nikon, Ti-E Eclipse) equipped with a 100× 1.49 N.A. oil immersion objective (Nikon, Plano Apo). The xy position of the stage was controlled by ProScan linear motor stage controller (Prior). The microscope was equipped with a MLC400B laser launch (Agilent), with 405 nm, 488 nm, 561 nm and 640 nm laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying CCD camera (Andor Technology, iXon Ultra 888). Illumination and image acquisition is controlled by NIS Elements Advanced Research software (Nikon).
Mlc400b laser launch
Manufactured by Agilent Technologies
The MLC400B laser launch is a compact and versatile device designed to launch laser beams into optical fibers. It provides a stable and controlled environment for coupling laser light into fiber optic transmission systems. The core function of the MLC400B is to efficiently launch laser light into optical fibers, enabling the transfer of optical signals.
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5 protocols using mlc400b laser launch
1
Multicolor Fluorescence Microscopy Imaging
2
High-resolution Fluorescence Microscopy Setup
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Imaging was performed with an inverted microscope (Ti-E Eclipse; Nikon) equipped with a 100× 1.49 NA oil immersion objective (Plano Apo; Nikon). The xy position of the stage was controlled by ProScan linear motor stage controller (Prior). The microscope was equipped with an MLC400B laser launch (Agilent) equipped with 405-nm (30 mW), 488-nm (90 mW), 561-nm (90 mW), and 640-nm (170 mW) laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying charge coupled device camera (iXon Ultra 888; Andor Technology). Illumination and image acquisition were controlled by NIS Elements Advanced Research software (Nikon).
3
Inverted Microscope Imaging Protocol
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Imaging was performed with an inverted microscope (Nikon, Ti-E Eclipse) equipped with a 100×1.49 N.A. oil immersion objective (Nikon, Plano Apo). The xy position of the stage was controlled by ProScan linear motor stage controller (Prior). The microscope was equipped with an MLC400B laser launch (Agilent) equipped with 405 nm (30 mW), 488 nm (90 mW), 561 nm (90 mW), and 640 nm (170 mW) laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying CCD camera (Andor Technology, iXon Ultra 888). Illumination and image acquisition was controlled by NIS Elements Advanced Research software (Nikon).
4
Inverted Fluorescence Microscopy Protocol
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Imaging was performed with an inverted microscope (Nikon, Ti-E Eclipse) equipped with a 100 × 1.49 N.A. oil immersion objective (Nikon, Plano Apo). The xy position of the stage was controlled by ProScan linear motor stage controller (Prior). The microscope was equipped with an MLC400B laser launch (Agilent) equipped with 405 nm (30 mW), 488 nm (90 mW), 561 nm (90 mW), and 640 nm (170 mW) laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying CCD camera (Andor Technology, iXon Ultra 888). Illumination and image acquisition were controlled by NIS Elements Advanced Research software (Nikon).
5
Temperature-controlled Microscopy Imaging
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Imaging was performed with an inverted microscope (Nikon, Ti-E Eclipse) equipped with a 100 3 1.49 N.A. oil immersion objective (Nikon, Plano Apo). The xy position of the stage was controlled by a Pro-Scan linear motor stage controller (Prior). The microscope was equipped with a MLC400B laser launch (Agilent), with 405 nm, 488 nm, 561 nm and 640 nm laser lines. The excitation and emission paths were filtered using appropriate single bandpass filter cubes (Chroma). The emitted signals were detected with an electron multiplying CCD camera (Andor Technology, iXon Ultra 888). Illumination and image acquisition was controlled by NIS Elements Advanced Research software (Nikon). Temperature-controlled motility assays were performed using a Linkam PE100-NIF inverted Peltier stage and T96 System Controller. Flow chamber slides were covered with a custom copper-plated aluminum fitting and empirical temperature of the flow chamber was monitored using a Type K thermocouple sensor probe.
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