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Vorinostat

Manufactured by Cell Signaling Technology
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Vorinostat is a histone deacetylase (HDAC) inhibitor. It functions by inhibiting the activity of HDAC enzymes, which are involved in the regulation of gene expression.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Trametinib, by Cell Signaling Technology (1 mentions) Azacitidine, by Merck Group (1 mentions) Itf 2357, by Apexbio (1 mentions) Tubastatin a, by Apexbio (1 mentions) Puromycin, by Merck Group (1 mentions) Vorinostat, by Selleck Chemicals (1 mentions) Acetyl h4 lys5, by Cell Signaling Technology (1 mentions) Histone h4, by Cell Signaling Technology (1 mentions) Acetyl h3 lys9, by Cell Signaling Technology (1 mentions) Histone h3, by Cell Signaling Technology (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)

4 protocols using vorinostat

1

Inhibiting pERK Production in Drosophila

Cited 2 times
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Two drugs known to inhibit pERK production (Trametinib and Vorinostat) were used by feeding as published previously [12 (link),45 (link),46 (link)]. Both Trametinib (Cell Signaling, 62206S) and Vorinostat (Cell Signaling, 12520S) were dissolved in dimethylsulfoxide (DMSO; Fisher, 67-68-5) at 15 mM and 20 mM, respectively, to create stock solutions. Both drugs were then added to Drosophila food yeast paste and in the standard cornmeal/agar/molasses food in the final concentrations of 0.5 mM (Trametinib) and 1 mM (Vorinostat). Drosophila were induced to lay eggs on selection apple juice plates with drugged yeast paste food. Hatching first instars were selected and placed in standard vials containing Trametinib, Vorinostat, or control food with DMSO only. Larvae were reared in a 12-hour light/dark cycling incubators at 25°C and then collected as wandering third instars for TEVC studies.
Leahy S.N., Song C., Vita D.J, & Broadie K. (2023). FMRP activity and control of Csw/SHP2 translation regulate MAPK-dependent synaptic transmission. PLOS Biology, 21(1), e3001969.
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2

HDAC Inhibitors Modulate EMT Markers

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Vorinostat (Vor) was obtained from cell signaling technology (Danvers, MA, USA) and romidepsin (Rom) from Selleck Chemicals. Stock solutions of Vorinostat and romidepsin were prepared in DMSO. Cell lines were seeded into 6-well plates and incubated overnight, cells were treated with HDAC inhibitors when they reached ~30 to 40% of confluency. For dose-dependent experiments, cells were treated with 2.5 µM, 5 µM and 10 µM of Vorinostat, and 5 nM, 10 nM, and 15 nM of romidepsin. To investigate changes in EMT markers, MDA-MB-231 and HS578T cells were treated 2.5 µM of Vorinostat and 5 nM of romidepsin. Following 24 h of incubation, RNA was collected and qPCR was performed. Three replicate wells were tested per concentration.
Makena M.R., Ko M., Dang D.K, & Rao R. (2021). Epigenetic Modulation of SPCA2 Reverses Epithelial to Mesenchymal Transition in Breast Cancer Cells. Cancers, 13(2), 259.
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3

Preparation of Epigenetic Drug Solutions

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Azacitidine (Sigma) was dissolved in PBS at 500uM (in vitro) and 7.1 mg/mL (in vivo), aliquoted, and stored at −80C for single use. ITF-2357 (Apexbio) was dissolved in DMSO to concentrations of: 1mM, 500uM, 250uM, 100uM, 50uM and 25uM (in vitro) and 50mg/mL (in vivo), aliquoted and stored at −20C. MS-275 (Syndax) was dissolved in DMSO to concentrations of: 2.5mM, 1mM, 500uM, 250uM, 125uM (in vitro) and 50mg/mL (in vivo), aliquoted and stored at −20C. MGCD0103 (Apexbio) was dissolved in DMSO to concentrations of: 1mM, 500uM, 250uM, 100uM, 50uM and 25uM (in vitro) and 10mg/mL (in vivo), aliquoted and stored at −20C. RGFP996 (Apexbio) was dissolved in DMSO to concentrations of: 10mM, 5mM, 2.5uM, 1uM, 500uM and 100uM (in vitro), aliquoted and stored at −20C. Tubastatin A (Apexbio) was dissolved in DMSO to concentrations of: 10mM, 5mM, 2.5mM, 1mM, 500uM and 250uM (in vitro), aliquoted and stored at −20C. Vorinostat (Cell signaling) was dissolved in DMSO to concentrations of 300uM (in vitro), aliquoted, and stored at −20C. Puromycin (Sigma), dissolved in PBS at 1mg/mL, aliquoted and stored −20C.
Topper M.J., Vaz M., Chiappinelli K.B., DeStefano Shields C.E., Niknafs N., Yen R.W., Wenzel A., Hicks J., Ballew M., Stone M., Tran P.T., Zahnow C.A., Hellmann M.D., Anagnostou V., Strissel P.L., Strick R., Velculescu V.E, & Baylin S.B. (2017). Epigenetic Therapy Ties MYC Depletion to Reversing Immune Evasion and Treating Lung Cancer. Cell, 171(6), 1284-1300.e21.
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4

Vorinostat's Effects on U87 GBM Cells

Cited 1 time
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U87 human GBM cells were supplied by the University of California San Francisco (UCSF) Brain Tumor Research Center Preclinical Therapeutics Core (20 (link),22 (link)). Cells were routinely fingerprinted by Cell Line Genetics using single nucleotide polymorphism within 6 months of any study. Cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, GIBCO) supplemented with 10% FBS, 2mM L-glutamine, 100units/mL penicillin, 100μg/mL streptomycin and maintained in 5% CO2 at 37°C.
Vorinostat (SAHA, MK0683) was purchased from Selleckchem. For all in vitro studies Vorinostat was prepared by dissolution in DMSO for a stock concentration of 10mM that was stored at −20°C. Cells were then treated for 48h either with Vorinostat diluted to a final concentration of 10μM (treated cells) or 1:1000 DMSO (controls) in culture medium. The effect of drug on cell number was assessed by seeding ~2×105 cells per flask, allowing the cells to adhere overnight, initiating treatment for 48h with either Vorinostat or DMSO, and then counting the number of cells per flask.
The effect of drug on acetylation (n=2 for both control and Vorinostat-treated) was assessed by immune-precipitation following the previously described method (26 (link)) using antibodies specific for the acetyl-H3-Lys9, acetyl-H4-Lys5, histone-H3, and histone-H4 (1:1000) (Cell Signaling Technology).
Radoul M., Najac C., Viswanath P., Mukherjee J., Kelly M., Gillespie A.M., Chaumeil M.M., Eriksson P., Santos R.D., Pieper R.O, & Ronen S.M. (2018). HDAC inhibition in glioblastoma monitored by hyperpolarized 13C MRSI.. NMR in biomedicine, 32(2), e4044.
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