Cell migration ability was tested using 8.0-μm-pore polycarbonate-membrane-inserted transwell chambers (Corning, New York, NY, United States). The polycarbonate membrane of the upper chamber was coated with 0.1% gelatin, before the upper chambers with siCONT- and siNEDD4-treated cells (5 × 104 for each chamber). After 3 h, the cells through the membrane were fixed with 4% paraformaldehyde for 5 min and stained with 1% crystal violet in 2% ethanol. Pictures of the stained cells were taken by microscopy (Revolve, ECHO, San Diego, CA, United States).
8.0 μm pore polycarbonate membrane inserted transwell chambers
Manufactured by Corning
Sourced in United States
The 8.0-μm-pore polycarbonate-membrane-inserted transwell chambers are a type of laboratory equipment used for cell culture and membrane permeability studies. The chambers consist of a porous polycarbonate membrane inserted into a well, allowing for the separation of two compartments. This design facilitates the study of cell migration, invasion, and transport across the membrane.
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Lab products found in correlation
2 protocols using 8.0 μm pore polycarbonate membrane inserted transwell chambers
1
Transwell Assay for Cell Migration
2
Transwell Invasion Assay
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Invasiveness ability was also tested using 8.0-μm-pore polycarbonate membrane-inserted transwell chambers (Corning, New York, NY, United States). The polycarbonate membrane of the upper chamber was coated with 0.3 mg/ml of matrigel, before the upper chambers with siCONT- and siNEDD4-treated cells (5 × 104 for each chamber). After 24 h, cells through the membrane were fixed with 4% paraformaldehyde for 5 min and stained with 1% crystal violet in 2% ethanol. Pictures of the stained cells were taken by microscopy (Revolve, ECHO, San Diego, CA, United States).
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