Anti usp8

Spautin-1 (S7888), Imatinib (S1026), and MG132 (S2619) were from Sellectchem (Houston, TX, USA). SKP2-C25 (M60136) was from Xcessbio Biosciences, Inc. (San Diego, CA). MTS reagent (G3582) was from Promega Corporation (Madison, WI, USA). Co-IP assay kit (14311D) was from Life Technologies (Carlsbad, CA). Antibodies: anti-Ubiquitin (#3936), anti-K63-Ubiquitin (#12930), anti-K48-Ubiquitin (#12805), anti-USP10 (#8501), anti-USP1 (#8033), anti-USP2 (#8036), anti-USP7 (#4833), anti-USP8 (#11832), anti-USP14 (#11931), anti-USP15 (#66310), anti-USP18 (#4813), anti-UCHL1 (#13179), anti-UCHL3 (#8141), anti-CYLD (#8462), anti-A20 (#5630), anti-SKP2 (#2652), anti-p27 (#3686), anti-FLAG (#14793), anti-HA (#3724), anti-phospho-c-Abl(Y245) (#2861), anti-c-Abl (#2862), anti-phospho-STAT5 (#9359), anti-STAT5 (#25656), anti-phospho-Crkl (#3181) and anti-Crkl (#38710) (Cell Signaling Technology, Beverly, MA, USA); anti-UCHL5 (ab124931), anti-USP13 (ab109264) (Abcam, Cambridge, MA); anti-GAPDH (BS60630) (Bioworld Technology, Inc., Louis Park, MN, USA).

The following primary antibodies were purchased from Cell Signaling Technology (Danvers, USA): anti-cleaved Notch1 (4147s; 1:1,000), anti-Hes1 (11988s; 1:1,000), p-eNOS (9570s, 1:1,000), eNOS (32027s, 1:1,000), iNOS (13120s,1:1,000), anti-AKT (4691s; 1:1,000), anti-phospho-AKT antibody (Ser473) (4060s; 1:1,000), anti-PTEN antibody (9559s; 1:1,000), anti-Actin antibody (4970s; 1:1,000), and anti-USP8 (11832s; 1:1,000). The following primary antibodies were obtained from Abcam (Waltham, USA): anti-USP11 (ab109232; 1:1,000), anti-VE-Cad antibody (ab33168; 1:1,000), and anti-Zo-1 antibody (ab216880; 1:1,000). LPS (L4516) and interleukin 6 (IL-6) were acquired from Sigma-Aldrich Merck KGaA (Germany). Melatonin and tangeretin (TGN) were purchased from Aladdin (purity >95%; Aladdin, China).