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Anti icos pe

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Anti-ICOS PE is a fluorochrome-conjugated antibody that binds to the inducible T cell costimulator (ICOS) receptor expressed on activated T cells. It is a useful tool for the identification and analysis of ICOS-expressing cells in flow cytometry applications.

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Lab products found in correlation

Anti cxcr5 apc, by BioLegend (2 mentions) Anti pd 1 pe cy7, by BioLegend (2 mentions) Anti igd pe, by BioLegend (2 mentions) Anti cd4 pe, by BioLegend (2 mentions) Anti cd19 pb, by BioLegend (2 mentions) Foxp3 intracellular staining kit, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Anti cd19 pe, by BioLegend (1 mentions) Anti cd4 fitc, by BioLegend (1 mentions) Anti cd138 pe, by Thermo Fisher Scientific (1 mentions) Anti cd40, by BioLegend (1 mentions) Anti pd 1 pe, by BioLegend (1 mentions) Anti cd3 fitc, by BioLegend (1 mentions) Anti cd4 percp cy5, by BioLegend (1 mentions) Anti cxcr5 allophycocyanin, by BioLegend (1 mentions) Anti cd3 percp cy5, by BioLegend (1 mentions) Anti cd19 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd80 bv421, by BioLegend (1 mentions) Anti cd19 bv605, by BioLegend (1 mentions) Anti cd40 fitc, by BD (1 mentions)

Close spelling variants of this product

PE-conjugated anti-ICOS (3 citations) ICOS-PE (3 citations) Anti-ICOS (9 citations)

4 protocols using anti icos pe

1

Phenotyping Lymphocyte Subsets in Mice

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Lymphocytes were purified from the spleen or lymph nodes of WT and Rgs1 KD mice and labelled with the following antibodies: anti-PD1 PE-Cy7, anti-CXCR5 APC, anti-CD4 PE, anti-IgD PE, anti-ICOS PE or anti-CD19 PB (BioLegend). Foxp3 intracellular staining kit (eBioscience) was used to label follicular regulatory T cells. Fixation/Permeabilization buffers were used to stain intracellular Bcl6 and Ki-67. Intrinsic GFP and Raspberry fluorophores were also analyzed in specific experiments. Data were acquired on an LSRII flow cytometer (BD Biosciences) and analyzed using the FlowJo software (TreeStar Inc.).
Caballero-Franco C, & Kissler S. (2016). The autoimmunity-associated gene RGS1 affects the frequency of T follicular helper cells. Genes and immunity, 17(4), 228-238.
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2

Immunophenotyping of Mouse and Human Cells

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For mouse cell phenotype analysis, anti CD3-percp/cy5.5, anti CD4-FITC, anti CXCR5-allophycocyanin, anti ICOS-PE, and anti PD-1-PE were purchased from BioLegend (San Diego, CA, USA). Anti CD19-FITC, anti CD138-PE, anti IgD-allophycocyanin, anti CD27-percp/cy5.5, and relevant IgG isotypes were purchased from eBioscience (San Diego, CA, USA).
For human cell analysis, anti CD3-FITC, anti CD4-percp/cy5.5, anti CXCR5-allophycocyanin, anti PD-1-PE, and anti CD19-PE were purchased from BioLegend. Anti-IL-21 neutralizing antibody (eBioscience) and anti-CD40 (BioLegend) were used for functional analysis in vitro.
Guo J., Zhao C., Wu F., Tao L., Zhang C., Zhao D., Yang S., Jiang D., Wang J., Sun Y., Li Z., Li H, & Yang K. (2018). T Follicular Helper-Like Cells Are Involved in the Pathogenesis of Experimental Autoimmune Encephalomyelitis. Frontiers in Immunology, 9, 944.
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3

Phenotyping Lymphocyte Subsets in Mice

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Lymphocytes were purified from the spleen or lymph nodes of WT and Rgs1 KD mice and labelled with the following antibodies: anti-PD1 PE-Cy7, anti-CXCR5 APC, anti-CD4 PE, anti-IgD PE, anti-ICOS PE or anti-CD19 PB (BioLegend). Foxp3 intracellular staining kit (eBioscience) was used to label follicular regulatory T cells. Fixation/Permeabilization buffers were used to stain intracellular Bcl6 and Ki-67. Intrinsic GFP and Raspberry fluorophores were also analyzed in specific experiments. Data were acquired on an LSRII flow cytometer (BD Biosciences) and analyzed using the FlowJo software (TreeStar Inc.).
Caballero-Franco C, & Kissler S. (2016). The autoimmunity-associated gene RGS1 affects the frequency of T follicular helper cells. Genes and immunity, 17(4), 228-238.
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4

Comprehensive Immunophenotyping of Immune Cells

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Anti-CD19 APC-Cy7, anti-CD38 PerCp-Cy5.5, anti-CD4 PE-Cy7, anti-CD25 PerCP-Cy5.5, anti-CD86 APC, anti-CD80 BV421, anti-CD45RA BV605, anti-ICOS PE, anti-CD21 PE-Cy7, anti-CD80 BV421, anti-CD27 PerCp-Cy5.5, anti-PD-1 APC, anti-CD45RA APC-Cy7, anti-HLA-DR BV605, anti-CD11c AF700, anti-CD86 BV711, anti-CD95 BV650, anti-IgD BV786, anti-FCRL5 APC, anti-CXCR3 BV421, anti-CCR6 BV605, anti-CD19 BV650, anti-CD19 BV605, anti-PD-L1 BV421, anti-PD-L2 PE, anti-ICOS-L PE, anti-OX40L PE, anti-IL-10 PE, anti-IL-10 APC, anti-TNF-α APC-Cy7 (all obtained from BioLegend); anti-IgD FITC and anti-IgD PE (both from Southern Biotech); anti-CD27 PE (Dako); and anti-CD21 PE-Cy7, anti-CD69 FITC, anti-HLA-DR FITC, anti-CD27 BV605, anti-CD40 FITC, anti-CD40L PE, anti-ICAM-1 PE, anti-CXCR5 BUV395 and anti-CD27 BUV395 (all from BD Biosciences).
Reincke M.E., Payne K.J., Harder I., Strohmeier V., Voll R.E., Warnatz K, & Keller B. (2020). The Antigen Presenting Potential of CD21low B Cells. Frontiers in Immunology, 11, 535784.
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