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2 protocols using malondialdehyde analyzing kit

1

Oxidative Stress Biomarkers Analysis

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Salidroside, ketamine, primary antibody against β-actin, and hematoxylin and eosin were supplied by Sigma Chemical Company (St. Louis, MO, USA). A malondialdehyde analyzing kit was obtained from Nanjing Jiancheng Institute of Bioengineering (Nanjing City, China). Protein quantification kit was purchased from Bio-Rad Laboratories (Hercules, CA, USA). The primary antibodies against copper- and zinc-containing (Cu-Zn) superoxide dismutase and catalase, horseradish peroxidase-labelled secondary antibody, and an enhanced chemiluminescence kit were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). The other chemicals used in the course of study were high-quality and commercially obtainable.
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2

Sesamol-Based Anti-Oxidant Evaluation

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Sesamol, anti-β-actin antibody, ketamine, and hematoxylin and eosin were procured from Sigma Chemical Company (St. Louis, MO, USA). A protein quantification kit was from Bio-Rad Laboratories (Hercules, CA, USA). The anti-cAMP-responsive element modulator-τ (CREMτ) antibody, horseradish peroxidase-conjugated secondary antibody, and enhanced chemiluminescence detection reagents were supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA). A malondialdehyde analyzing kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing City, China). Other reagents used in the study were high grade and commercially available.
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