Viral prep 51507 aavrg

Manufactured by Addgene

Sourced in United States

Viral prep # 51507-AAVrg is a lab equipment product designed for the preparation of adeno-associated virus (AAV) vectors. The product enables the purification and concentration of AAV particles, which are commonly used in gene delivery and gene therapy applications.

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Lab products found in correlation

Aavrg cag gfp, by Addgene (1 mentions) Aavrg pmsyn1 ebfp cre, by Addgene (1 mentions) Clozapine n oxide, by Biosynth (1 mentions)

2 protocols using viral prep 51507 aavrg

Viral Vector-Mediated Genetic Manipulation

Cited 1 time

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AAV8-hSyn-Cre (titer: 6.5 × 10¹² molecules/ml) was sourced from the UNC Vector Core (Chapel Hill, NC, USA). AAVrg-CAG-GFP (titer: 5 × 10¹² vg/mL, this construct was a gift from Edward Boyden to Addgene- viral prep # 37825-AAVrg) and AAVrg pmSyn1-EBFP-Cre (titer: 6 × 10¹² vg/mL, this construct was a gift from Hongkui Zeng to Addgene -viral prep # 51507-AAVrg; Madisen et al., 2015 (link)), were sourced from Addgene (MA, USA). All constructs were administered 1 ul bilaterally and a minimum of 3 weeks incubation was allowed.
For CaMKIIα cell-specific knockdown and fear conditioning and forced swim studies, 0.1 ul of AAV9.CamKII.HI.eGFP-Cre.WPRE.SV40 (CaMKIIα Cre) [Penn Vector Core] (titer: 6.544 × 10¹³ diluted to 6.544 × 10¹¹] was injected bilaterally and allowed 5 weeks to incubate.

Scheimann J.R., Moloney R.D., Mahbod P., Morano R.L., Fitzgerald M., Hoskins O., Packard B.A., Cotella E.M., Hu Y.C, & Herman J.P. (2019). Conditional deletion of glucocorticoid receptors in rat brain results in sex-specific deficits in fear and coping behaviors. eLife, 8, e44672.

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Methodological Approach to Modulate mPFC-AcbSh Pathway

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A retrogradely-transported adeno-associated virus carrying Cre recombinase (AAV pmSyn1-EBPF-Cre, abbreviated retro-Cre; Addgene viral prep #51507-AAVrg; a gift from Hongkui Zeng) was injected into the medial shell of the nucleus accumbens (AcbSh). In order to specifically target the mPFC to AcbSh pathway, Cre-dependent DREADD constructs were injected into the mPFC, a well-defined source of afferent input to the AcbSh. These were either excitatory [pAAV-hSyn-DIO-hM3D(Gq)-mCherry, abbreviated hM3Dq; Addgene viral prep #50474-AAV5], inhibitory [pAAV-hSyn-DIO-hM4D(Gi)-mCherry, abbreviated hM4Di; UNC Vector Core, AAV5] or contained only the mCherry fluorophore as a control (pAAV-hSyn-DIO-mCherry, abbreviated mCherry; Addgene viral prep #50459-AAV5; a gift from Bryan Roth). The activity of the mPFC-AcbSh pathway was then modulated using the synthetic ligand clozapine N-oxide (CNO; Carbosynth Ltd, Berkshire UK). CNO was dissolved in 5 % DMSO, diluted in 0.9 % saline and administered intraperitoneally to all animals (hM3Dq; 0.3 mg/kg, hM4Di; 3.0 mg/kg. mCherry; both doses, counterbalanced) 30 min prior to the onset of the feeding period or behavioral intervention and 90 min prior to transcardial perfusion.

Milton L.K., Mirabella P.N., Greaves E., Spanswick D.C., van den Buuse M., Oldfield B.J, & Foldi C.J. (2021). Suppression of Corticostriatal Circuit Activity Improves Cognitive Flexibility and Prevents Body Weight Loss in Activity-Based Anorexia in Rats. Biological psychiatry, 90(12).

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