Rtpespa150

The atomic force microscopy (AFM) was performed to determine the intermolecular interactions and the degree of components’ aggregation in layers of the free components (Ber and C₆₀) and their nanocomplex (C₆₀-Ber). AFM measurements were done with the “Solver Pro M” system (NT-MDT, Moscow, Russia). A drop of investigated solution was transferred on the atomically smooth substrate to deposit layers. Measurements were carried out after complete evaporation of the solvent. A freshly broken surface of mica (SPI supplies, V-1 grade) was used as a substrate. Measurements were carried out in a semicontact (tapping) mode with AFM probes of the RTPESPA150 (Bruker, 6 N/m, 150 kHz) type.

The highly stable pristine C₆₀ fullerene aqueous colloid solution (C₆₀FAS; purity > 99.5%, concentration 0.15 mg/ml) was prepared according to the method of [19 (link), 20 (link)]. Briefly, it is based on the transferring of C₆₀ fullerenes from toluene to an aqueous phase with the help of ultrasonic treatment.
The atomic force microscopy (AFM) was performed to determine the size of C₆₀ fullerene particles (their aggregates) in the aqueous solution. Measurements were done with the “Solver Pro M” system (NT-MDT Spectrum Instruments, Russia). A drop of investigated solution was transferred on the atomic-smooth substrate to deposit layers. Measurements were carried out after complete evaporation of the solvent. A freshly broken surface of mica (V-1 grade, SPI supplies, West Chester, PA, USA) was used as a substrate. Measurements were carried out in a semicontact (tapping) mode with AFM probes of the RTPESPA150 (Bruker, 6 N/m, 150 kHz) type.
We used the dynamic light scattering (DLS) and zeta potential measurements for ascertaining the hydrodynamic size and electrokinetic potential of the prepared C₆₀FAS. The tested volume of C₆₀FAS was 1.5 ml. Measurements were conducted on a Zetasizer Nano ZS90 (Malvern, Malvern, Worcestershire, UK) at 25°C. Results were evaluated using the Smoluchowski approximation, which is known to be rigorously valid only for spherical-like particles.

The atomic force microscopy (AFM) and scanning tunneling microscopy (STM) were performed to determine the size of C₆₀ fullerene particles (their aggregates) in an aqueous solution. Measurements were done with the “Solver Pro M” system (NT-MDT, Moscow, Russia). A drop of investigated solution was transferred to the atomic-smooth substrate to deposit layers. Measurements were carried out after complete evaporation of the solvent. For AFM studies, a freshly broken surface of mica (SPI supplies, V-1 grade) was used as a substrate. Measurements were carried out in a semicontact (tapping) mode with AFM probes of the RTPESPA150 (Bruker, 6 N/m, 150 kHz) type. STM studies were performed with the Au (111) surface obtained after annealing substrates of Au/mica (Phasis, Geneva, Switzerland) in a gas burner flame (propane-butane). The typical tunneling current and voltage values were 0.027–0.1 nA and 0.1–1 V, respectively.