Impact 2 qtof ms

Manufactured by Bruker

Sourced in Germany

The Impact II QTOF-MS is a high-performance mass spectrometry instrument manufactured by Bruker. It is a quadrupole time-of-flight mass spectrometer that provides accurate mass measurements and high-resolution analysis of a wide range of analytes. The core function of the Impact II QTOF-MS is to perform precise and sensitive detection and identification of molecular compounds.

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Lab products found in correlation

1290 infinity 2 uhplc, by Bruker (3 mentions) Eclipse plus c18 column, by Agilent Technologies (1 mentions) Facsaria 2, by BD (1 mentions) Csh c18 column, by Waters Corporation (1 mentions) Tasq software, by Bruker (1 mentions) Compass dataanalysis 4, by Bruker (1 mentions) Plrp s column, by Agilent Technologies (1 mentions) 1290 infinity uhplc, by Agilent Technologies (1 mentions) Apollo 2 esi source, by Bruker (1 mentions) Elute uplc system, by Bruker (1 mentions) Impact 2 esi q tof system, by Bruker (1 mentions)

Spelling variants of this product

Impact II (78 citations) Impact II QTOF (34 citations) QTOF MS (9 citations) Impact qTOF-MS (2 citations) Impact II MS (2 citations)

6 protocols using impact 2 qtof ms

Tracing Cellular Lipid Metabolism

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Cells (3 × 10⁵) were cultured in RPMI 1640, supplemented with 10 mM ¹³C-palmitate for 18 h, after which they were rinsed with cold 0.9% NaCl and extracted using 0.2 mL of 80% MeOH on dry ice, and dried using a SpeedVac. Label tracing was carried out using an Agilent 1290 Infinity II UHPLC inline with a Bruker impact II QTOF-MS operated in full scan (MS1) mode. LC parameters were identical to those used for targeted metabolite quantification. Data processing including correction for natural isotope abundance was performed by an in-house R script. Metabolite peaks were identified based on exact mass and matching of retention time to a pure standard.

Castoldi A., Monteiro L.B., van Teijlingen Bakker N., Sanin D.E., Rana N., Corrado M., Cameron A.M., Hässler F., Matsushita M., Caputa G., Klein Geltink R.I., Büscher J., Edwards-Hicks J., Pearce E.L, & Pearce E.J. (2020). Triacylglycerol synthesis enhances macrophage inflammatory function. Nature Communications, 11, 4107.

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Lipidomic Analysis by UHPLC-QTOF-MS

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The extraction was done using the same method described on Lipidomics procedure, above. Data were acquired by LC–MS using an Agilent 1290 Infinity II UHPLC inline with a Bruker Impact II QTOF-MS operating in negative and positive ion mode (two injections per sample). Scan range was from 50 to 1600 Da. Mass calibration was performed at the beginning of each run. LC separation was on a Zorbax Eclipse plus C18 column (100 × 2 mm, 1.8 µm particles) using a solvent gradient of 70% buffer A (10 mM ammonium formiate in 60:40 acetonitrile:water) to 97% buffer B (10 mM ammonium formiate in 90:10 2-propanol:acetonitrile). Flow rate was from 400 µL min⁻¹, autosampler temperature was 5 °C and injection volume was 2 µL. Data processing was carried out using Bruker MetaboScape 4 software. Fatty acid composition of TGs and Phospholipids was determined by matching measured spectra to reference spectra from LipidBlast. Peak height was used for relative quantification.

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Metabolomic Profiling of 10x-25x10^4 ResKCs

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ResKCs (10x10⁴ - 25x10⁴) were sorted in DMEM supplemented with 10% FCS (Bodinco) using a BD FACSARIA II and ARIA III. Cells were then incubated for 30 min at 37°C, before being pelleted at -9°C and washed in 1ml ice cold NaCl (0.9%). Cells were then pelleted at -9°C and snap frozen in liquid nitrogen. Samples were than extracted in 100uL of 30:50:20 acetonitrile:methanol:milli-Q H₂O pre-cooled at -20°C, dried using speed vac and resuspended in 20uL of 30:50:20 acetonitrile:methanol:milli-Q H₂O for metabolites that are identified in negative ionization mode (polar), or in H₂O for metabolites that are identified in positive ionization mode (nonpolar). Metabolite quantification was carried out using Agilent 1290 Infinity II UHPLC in line with a Bruker impact IIQTOF-MS operated in full scan (MS1) mode. LC separation was performed on a Waters CSH-C18 column (100 × 2.1 mm, 1.7 μm particles) using a binary solvent gradient of 100% buffer A (0.1% formic acid in water) to 97% buffer B (50:50 methanol:acetonitrile). Flow rate was 400 μL/min, autosampler temperature was 4°C, and injection volume was 3 μL. Data processing was performed using TASQ™ Software (Target Analysis for Screening and Quantification) (Bruker Daltonics Inc.).

Remmerie A., Martens L., Thoné T., Castoldi A., Seurinck R., Pavie B., Roels J., Vanneste B., De Prijck S., Vanhockerhout M., Binte Abdul Latib M., Devisscher L., Hoorens A., Bonnardel J., Vandamme N., Kremer A., Borghgraef P., Van Vlierberghe H., Lippens S., Pearce E., Saeys Y, & Scott C.L. (2020). Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver. Immunity, 53(3), 641-657.e14.

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Quantitative Label Tracing via LC-MS

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Label tracing by LC-MS was carried out using an Agilent 1290 Infinity II UHPLC inline with a Bruker Impact II QTOF-MS operating in negative ion mode. Scan range was from 50 to 1600 Da. Mass calibration was performed at the beginning of each run. LC separation was on a Zorbax Eclipse plus C18 column (100 × 2 mm, 1.8 μm particles) using a solvent gradient of 70% buffer A (10 mM ammonium formiate in 60:40 acetonitrile:water) to 97% buffer B (10 mM ammonium formiate in 90:10 2-propanol:acetonitrile). Flow rate was from 400 μL/min, autosampler temperature was 5°C and injection volume was 2 μL. Data processing including correction for natural isotope abundance was carried out by an in-house R script.

Corrado M., Edwards-Hicks J., Villa M., Flachsmann L.J., Sanin D.E., Jacobs M., Baixauli F., Stanczak M., Anderson E., Azuma M., Quintana A., Curtis J.D., Clapes T., Grzes K.M., Kabat A.M., Kyle R., Patterson A.E., Geltink R.K., Amulic B., Steward C.G., Strathdee D., Trompouki E., O’Sullivan D., Pearce E.J, & Pearce E.L. (2020). Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity. Cell Metabolism, 32(6), 981-995.e7.

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Intact Protein Mass Analysis by LC-MS

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Intact-protein mass analysis was performed on a 1290 Infinity UHPLC (Agilent Technologies, Waldbronn, Germany), coupled to an Impact II QTOF-MS with Apollo II ESI source (Bruker Daltonics, Bremen, Germany). Reversed-phase separation was performed on a PLRP-S column (Agilent Technologies), with 5 µm particle size, 1000 Å pore size, 1 mm inner diameter, and 50 mm length. Mass spectra were recorded in positive ion ESI mode and the mass range from 600 to 7000 m/z (Details of LC-MS method in Supplementary Material). Data were analyzed in a Compass Data analysis 4.4 (Bruker Daltonics) tool. A weighted average of DEPC label incorporation was calculated for each sample. The weighted average of labels was calculated using the following formula:

Average label number = \frac{\sum_{x = 1}^{n} x I_{x}^{M o d i f i e d}}{\sum_{x = 1}^{n} I_{x}^{M o d i f i e d} + I_{0}^{U n m o d i f i e d}}

where x is the number of DEPC modifications, I₀ is the intensity of unlabeled peaks, and I_x is the intensity of labeled peaks [35 (link)].

Gaikwad M., Richter F., Götz R., Dörrbaum A., Schumacher L., Tonillo J., Frech C., Kellner R, & Hopf C. (2023). Site-Specific Structural Changes in Long-Term-Stressed Monoclonal Antibody Revealed with DEPC Covalent-Labeling and Quantitative Mass Spectrometry. Pharmaceuticals, 16(10), 1418.

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Compound Identification using Bruker Q-TOF

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A Bruker Daltonik Impact II ESI-Q-TOF System with a Bruker Daltonik Elute UPLC system (Bremen, Germany) was used to screen the compounds of interest in the extracts. The extract samples were dissolved in 2 ml DMSO and made up to 50 ml using acetonitrile. Then, the samples were centrifuged at 4,000 rpm for 2 min and transferred to autosampler where 3 μL was injected (59 standards were used to identify ms/z and the retention time).
After chromatographic separation, high-resolution Bruker TOF MS was utilized to determine m/z and the exact retention duration of each analyte. The Ion Source Apollo II ion Funnel electrospray source was used to power this instrument. The capillary voltage was 2,500 V, the nebulizer gas pressure was 2 bar, the dry gas (nitrogen) flow rate was 8 L/min, and the dry temperature was 200°C. The mass resolution was 50,000 FSR, and the mass accuracy was one ppm (Full Sensitivity Resolution). The repetition rate of the TOF was up to 20 kHz. Chromatographic separation was achieved using an Elute UHPLC connected to a Bruker Impact II QTOFMS.

Al Safi M.A., Rashid H.M., Afifi F.U, & Talib W.H. (2022). Gaz Alafi: A Traditional Dessert in the Middle East With Anticancer, Immunomodulatory, and Antimicrobial Activities. Frontiers in Nutrition, 9, 900506.

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