2 2 azino bis 3 ethylbenzthiazoline 6 sulphonic acid abts

Folin–Ciocalteu’s phenol reagent (cat# 47742); 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) (cat# 10102946001), O-Phenanthroline (cat# 516705) and the Griess reagent (cat# 516705) were purchased from Sigma (St Louis, MO, USA). Griess reagent, ACROS Organics (cat# AC328670500); Sodium persulfate (cat# AC202025000) and 1, 10-Phenanthroline (cat# P69100) were purchased from Fisher Scientific (Pittsburgh, PA, USA). All purchased chemicals were used without further purifications.

Trolox (97%), ethanol (99.5%), vanillin (99%), acid acetic (99.5%), gallic acid (97%), and perchloric acid (99.99%) were procured from Anpha Chemika, India. Other reagents, such as 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and Folin–Ciocalteu, were from Sigma (St. Louis, MO, U.S.A.). Additionally, α-amylase solution (ex-porcine pancreas, EC 3.2.1.1) and collagenase were purchased from Sigma. All the solvents and reagents used in this study were of analytical grade.

Okra fruits of Abelmoschus esculentus cv. Wufu used in this study were harvested from Chengdu, Sichuan Province, China. Monosaccharide standards, 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), sodium nitroprusside (SNP), vitamin C (Vc), griess reagent, lipopolysaccharide (LPS), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Loganic acid (LA), loganin (L) quercetin 3-O-glucoside (Q-glc), kaempferol 3-O-glucoside (Kf-glc), and cyanidin 3-O-glucoside (Cy-glc) were purchased from Extrasynthese (Genay, France). Further, 2,2-diphenyl-1-picrylhydrazyl (DPPH^•) ferrous chloride, tripyridyltriazine (TPTZ), potassium persulfate, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS^•+), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), and gallic acid were obtained from Sigma Chemical Co. (Steinheim, Germany). Methanol, acetonitrile, formic acid, and hydrochloric acid were obtained from POCh (Gliwice, Poland). All chemicals and solvents were of analytical grade.

Trolox (97%), ethanol (99.5%), vanillin (99%), acid acetic (99.5%), gallic acid (97%), and perchloric acid (99.99%) were procured from Anpha Chemika, India. Other reagents, such as 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and Folin–Ciocalteu, were from Sigma (St. Louis, MO, U.S.A.). Additionally, α-amylase solution (ex-porcine pancreas, EC 3.2.1.1) and collagenase were purchased from Sigma. All the solvents and reagents used in this study were of analytical grade.

Soybean phosphatidylcholine (SPC, purity > 90%) was a gift from Lipoid (Ludwigshafen, Germany). Imiquimod (purity >98%) was a gift from Laboratorio Lazar (Buenos Aires, Argentina). MONTANIDE™ ISA 763 A VG was from Seppic (Puteaux, France). Ficoll was from GE Healthcare (Munich, Germany). Hypaque was from Winthrop Products (Buenos Aires, Argentina). Roswell Park Memorial Institute (RPMI) 1640 medium was from Gibco, Life Technologies (New York, USA). Antibiotic/antimycotic solution (penicillin 10,000 IU/mL, streptomycin sulfate 10 mg/mL, amphotericin B 25 μg/mL), glutamine, and trypsin/ethylenediamine tetraacetic acid were from PAA Laboratories GmbH (Pasching, Austria). Fetal bovine serum (FBS) was from Internegocios, Cordoba, Argentina. Concanavalin A (ConA), Phorbol 12-myristate 13-acetate (PMA), Laurdan, saponin, Carboxy fluorescein succimidil ester (CFSE), 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) were from Sigma-Aldrich (San Diego, USA). The other reagents were analytic grade from Anedra, Research AG (Buenos Aires, Argentina).

Reagents were purchased from Merck (Darmstadt, Germany), except when otherwise mentioned. The chemicals including quercetin, Trolox, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) were purchased from Sigma (St. Louis, MO, USA). Dulbecco's Modified Eagle Medium (DMEM), Dulbecco's Modified Eagle Medium/Nutrient Mixture F12 (DMEM/F12), fetal bovine serum (FBS), and antibiotics were obtained from Gibco (Waltham, MA, USA).