Alexa fluor 555 conjugated anti mouse igm antibody

Manufactured by Southern Biotech

The Alexa Fluor 555-conjugated anti-mouse IgM antibody is a fluorescently labeled antibody that binds to mouse immunoglobulin M (IgM). Alexa Fluor 555 is a fluorescent dye that can be detected using common fluorescence detection methods.

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Lab products found in correlation

Superfrost plus gold slides, by Thermo Fisher Scientific (1 mentions) Prolong gold anti fade solution with dapi, by Thermo Fisher Scientific (1 mentions)

2 protocols using alexa fluor 555 conjugated anti mouse igm antibody

Immunofluorescence Imaging of FALCs

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Pictures of cell culture were taken on a Leica DM IL microscope with a SPOT RT3 camera (Spot Imaging). For confocal microscopy images of FALCs, recipient NSG were euthanized, and omentum and mesenteric fat tissues were dissected. The samples were prepared as described previously (Benezech et al., 2015 (link)) and stained with 1:200 Alexa Fluor 555-conjugated anti-mouse IgM antibody (SouthernBiotech). Confocal images were taken using an Olympus II microscope with UApoN340 20×/0.7W objectives (Olympus). ImageJ software was used to adjust and output the images.

Lin Y., Kobayashi M., Azevedo Portilho N., Mishra A., Gao H., Liu Y., Wenzel P., Davis B., Yoder M.C, & Yoshimoto M. (2019). Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis. Stem Cell Reports, 12(3), 572-583.

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Multicolor Immunofluorescence Imaging of Adipose Tissue

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Fat tissues were removed from the peritoneal cavity and fixed with 4% paraformaldehyde for 1 hour at 4°C. The samples were stained with 1:200 Alexa Fluor 555 conjugated anti-mouse IgM antibody (SouthernBiotech) and FITC conjugated anti-CD31 antibody or FITC-conjugated anti-CD11b antibody in 0.1% triton X100-PBS for 1 hour at room temperature. Then Alexa Fluor 555⁺ (red) areas were dissected out under a Leica™ mz9.5 fluorescent stereomicroscope and then mounted on Superfrost™ Plus Gold Slides (Thermo Fisher Scientific) with ProLong^® Gold Antifade solution with DAPI (Thermo Fisher Scientific). Confocal images were taken using an Olympus II microscope with UApoN340 20x/0.7W objectives (Olympus). ImageJ software was used to adjust and output the images.

Kobayashi M., Lin Y., Mishra A., Shelly C., Gao R., Reeh C.W., Wang P.Z., Xi R., Liu Y., Wenzel P., Ghosn E., Liu Y, & Yoshimoto M. (2020). Bmi1 maintains the self-renewal property of innate-like B lymphocytes. Journal of immunology (Baltimore, Md. : 1950), 204(12), 3262-3272.

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