Hyperpolarization of [1‐13C]pyruvic acid (Cambridge Isotope Laboratories, Inc., Andover, MA, USA) was performed using a home built 4.6 T pre‐polarizer. Neat [1‐13C]pyruvic acid was doped with trityl OX063 radical (15 mM) and ProHance® (1 mM) and was polarized at 128.915 GHz for 1.5 h at 1.15 K. Microwave irradiation was turned off and the sample was rapidly dissolved with 4 mL of superheated 100 mM phosphate buffer (pH 7.4) and 3 mL of the solution was transferred into a beaker near the fringe field (~ 5 G) of a GE 3 T 750 W. Polarization was measured as 10–12% at the time of injection. The final concentration of the HP pyruvate was 80 mM.
1 13c pyruvic acid
Manufactured by GE Healthcare
Sourced in United Kingdom
[1‐13C]pyruvic acid is a stable isotope-labeled compound used as a precursor in various metabolic studies and research applications. It consists of a pyruvic acid molecule with a carbon-13 isotope at the first carbon position. This compound serves as a tracer to investigate metabolic pathways and can be utilized in a range of analytical techniques, such as nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry.
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2 protocols using 1 13c pyruvic acid
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Hyperpolarized [1-13C]Pyruvic Acid Production
2
Hyperpolarized [13C]Pyruvate Dissolution
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The sample contained 44 mg [1‐13C]pyruvic acid (CIL, MA), 15 mM OXØ63 (GE Healthcare, Amersham, UK), and 1.4 mM gadoterate meglumine (Dotarem; Guerbet, Roissy, France) and was hyperpolarized using a Hypersense Polarizer (Oxford Instruments, Abingdon, UK) at 1.2 K in a magnetic field of 3.35 Tesla (T), with microwave irradiation at 94.126 GHz. The sample was then rapidly dissolved in 6‐mL buffer containing 40 mM Tris, 185 mM NaOH, and 100 mg/L EDTA heated to 180 °C and pressurized to 10 bar.
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