OT-I transgenic mice, C57BL/6, and Balb/c mice (6–7 weeks old) were supplied by Vital River Laboratory (Beijing, China). CEMIP knockdown (CEMIPKD), overexpression (CEMIPOE), and scramble MC38 (3×105) or CT26 (5×105) cells were subcutaneously injected into the right flanks of these mice. Mice were monitored for tumor growth every 2 days afterward. Tumor size was calculated using the formula (width2×length)/2. For chlorpromazine (MedChemExpress, USA) and chloroquine (MedChemExpress, USA) treatment, mice respectively received intraperitoneal injections of chlorpromazine (10 mg/kg), chloroquine (60 mg/kg), or phosphate-buffered saline (PBS) one time per day for the duration of the experiment. For ICB experiments, mice were intraperitoneally injected with anti-mouse programmed cell death protein-1 (PD-1) antibody (200 µg/per mouse) and anti-mouse cytotoxic T-lymphocytes-associated protein 4 (CTLA-4) antibody (100 µg/per mouse) or IgG1 isotype monoclonal antibodies on day 3 after tumor cell inoculation and then every 3 days for the duration of the experiment.
Chlorpromazine
Manufactured by MedChemExpress
Sourced in United States
Chlorpromazine is a chemical compound commonly used as a laboratory reagent. It serves as a phenothiazine-based antipsychotic medication and is a central nervous system depressant. The core function of Chlorpromazine is to act as a pharmaceutical ingredient for research and development purposes.
Automatically generated - may contain errors
Lab products found in correlation
Dynasore, by MedChemExpress (2 mentions)
C57bl 6, by Charles River Laboratories (1 mentions)
Balb c mice, by Charles River Laboratories (1 mentions)
Chloroquine, by MedChemExpress (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Penicillin and streptomycin, by Merck Group (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Sicxcr4, by RiboBio (1 mentions)
Harmine, by Abcam (1 mentions)
Pitstop 2, by MedChemExpress (1 mentions)
Mbx2982, by MedChemExpress (1 mentions)
5 n ethylcarboxamidoadenosine, by Merck Group (1 mentions)
α msh, by Apexbio (1 mentions)
Synergy 4, by Agilent Technologies (1 mentions)
Amiloride, by MedChemExpress (1 mentions)
Methyl β cyclodextrin, by MedChemExpress (1 mentions)
5 protocols using chlorpromazine
1
In Vivo Tumor Growth Evaluation
2
Nanomedicine Delivery for Ovarian Cancer
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Pristine MWNTs (purity at 95%, length between 0.5 and 2.0 um, and diameter between 4 and 6 nm) were obtained from XFNANO Materials (Nanjing, China). CD44v6 monoclonal antibody was purchased from Abcam (Cambridge, MA, United States). Gemcitabine (Gem), oxaliplatin (Oxa), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), agarose, coumarin C6, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (United States). Lipofectamine 2000 was obtained from Invitrogen (Carlsbad, CA, United States). The inhibitors used in this study (MβCD, dynasore, chlorpromazine) were purchased from MedChemExpress (MCE, South Brunswick Township, NJ, United States). The ovarian cancer cell line SW626 MG and SKOV-3 were purchased from American Type Cell Culture (ATCC, United States). Small interfering RNA (siRNA) targeting CXCR4 (siCXCR4) was designed and synthesized by RiboBio (Guangzhou, China). Cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) added with 1% penicillin and streptomycin (MO, Sigma) and fetal bovine serum (FBS, 10%, Gibco), and maintained in 37°C incubator filled with 5% CO2.
3
Detailed Protocols for Inhibitor Assays
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For the function analysis assay, DYRK1 inhibitors (AZ191, 40 μM; Harmine, 40 μM; Mirk-IN-1, 160 μM) or endocytosis inhibitors (Chlorpromazine, 10 μM; Pitstop2, 5 μM; Dynasore, 80 μM) were added separately at 17 hpf, and up to 23 hpf embryos were collected and stained with phalloidin for imaging. For the dextran dye uptake assay, DYRK1 inhibitor (AZ191, 40 μM; Harmine, 40 μM) was separately added at 16 hpf, and dextran was added at 18 hpf, and the whole embryo was bathed in a dye solution. Up to 26 hpf embryos were collected and fixed.
For the 4D Label-free Quantitative Phosphoproteomics assay, AZ191 and DMSO were separately added at 17 hpf, and up to 23 hpf embryos were collected and stored in liquid nitrogen for phosphorylation sequencing. AZ191, Mirk-IN-1, Chlorpromazine, Dynasore, and Pitstop2 were purchased from MedChemExpress, and Harmine was purchased from Abcam.
For the 4D Label-free Quantitative Phosphoproteomics assay, AZ191 and DMSO were separately added at 17 hpf, and up to 23 hpf embryos were collected and stored in liquid nitrogen for phosphorylation sequencing. AZ191, Mirk-IN-1, Chlorpromazine, Dynasore, and Pitstop2 were purchased from MedChemExpress, and Harmine was purchased from Abcam.
4
Pharmacological Agents for Biological Research
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Cabergoline was purchased from Abcam (Cambridge, UK). 5′-N-Ethylcarboxamidoadenosine was obtained from Sigma (Darmstadt, Germany). Isoproterenol (hydrochloride) was from Cayman (Ann Arbor, Michigan, USA). Chlorpromazine (hydrochloride) and MBX2982 was obtained from Medchemexpress (Monmouth Junction, NJ, USA). Neuromedin U-25 was purchased from Phoenix pharmaceuticals (Burlingame, CA, USA). α-MSH was obtained from APExBIO (Houston, TX, USA).
5
Endocytic Pathway Inhibitors in Peptide Uptake
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Chlorpromazine (CPZ), amiloride (AMI), and methyl-β-cyclodextrin (MCD) were purchased from MedChem Express. The cells were treated with different inhibitors (10 µM Chlorpromazine, 50 μM methyl-β-cyclodextrin, 20 µM amiloride) for 30 min at 37 °C. Subsequently, the cells were incubated with FITC-P-LPK for 2 h. The uptake of FITC-peptide was stopped by washing three times in ice-cold PBS. Then the fluorescence intensity was detected using a multi-well plate reader (BioTek synergy4) and observed by confocal laser scanning microscopy.
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