At GD 6, all pregnant dams received a subcutaneous injection of carprofen (5 mg/kg; Rimadyl, Zoetis, Florham Park, NJ) prior to surgery and were anesthetized with 3%–4% isoflurane (Iso-thesia, Abbott Laboratories, Abbott Park, IL) and maintained with 1%–3% isoflurane in 100% oxygen delivered by facemask. A 28-day osmotic minipump (ALZET Osmotic Pump Model 2ML4, DURECT Corp., Cupertino, CA) was inserted subcutaneously via a small incision between the scapulae and the incision was closed with wound clips. The minipump was filled with either sterile saline (control and MP only rats; Baxter Veterinary Supplies, Round Lake, IL) or nicotine tartrate (Sigma-Aldrich, St. Louis, MO) dissolved in sterile saline at a concentration sufficient to deliver 6 mg/kg/day (Nic only and Nic + MP rats). Supplemental heat was provided by insulated heating pads (SnuggleSafe, Lenric C21 Ltd. Littlehampton, West Sussex, UK) during anesthesia and recovery.
Isothesia
Manufactured by Abbott
Sourced in United States
Isothesia is a laboratory equipment product manufactured by Abbott. It is designed to maintain a constant temperature environment for various experimental and testing applications. The core function of Isothesia is to provide precise temperature control and monitoring capabilities to support accurate and reliable results in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Nicotine tartrate, by Merck Group (1 mentions)
Rimadyl, by Zoetis (1 mentions)
Alzet osmotic pump model 2ml4, by Durect (1 mentions)
Female swiss webster mice, by Charles River Laboratories (1 mentions)
Ketaset, by Zoetis (1 mentions)
Telazol, by Zoetis (1 mentions)
Vevo 770 high resolution imaging system, by Fujifilm (1 mentions)
Rmv 707b transducer, by Fujifilm (1 mentions)
5 protocols using isothesia
1
Nicotine Exposure During Gestation
2
Intranasal Toxin Application in Mice
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Swiss-Webster female
mice (22 to 25- g) were purchased from Charles
River Laboratories, Inc. (Wilmington, MA). Intranasal toxin application
was carried out by first lightly anesthetizing mice with isoflurane
(Isothesia, Abbott Laboratories North, Chicago, IL). Toxin was administered
by single application of 20 μL solution to the nares at doses
of 50-ng, 500-ng, and 5-μg. Care was taken to avoid generation
of aerosol during the administration of the toxin. The heads of animals
were maintained in an upright position to minimize drainage into the
posterior pharynx. Blood samples were collected at 30 min and 60 min
postintoxication via retro-orbital bleeding. Serum was separated by
centrifugation at 1665xg for 16 min, stored at −20 °C,
and shipped to Sandia National Laboratories for analysis. All procedures
involving animals were reviewed and approved by the Institutional
Animal Care and Use Committee, University of Massachusetts Dartmouth
(2003).
mice (22 to 25- g) were purchased from Charles
River Laboratories, Inc. (Wilmington, MA). Intranasal toxin application
was carried out by first lightly anesthetizing mice with isoflurane
(Isothesia, Abbott Laboratories North, Chicago, IL). Toxin was administered
by single application of 20 μL solution to the nares at doses
of 50-ng, 500-ng, and 5-μg. Care was taken to avoid generation
of aerosol during the administration of the toxin. The heads of animals
were maintained in an upright position to minimize drainage into the
posterior pharynx. Blood samples were collected at 30 min and 60 min
postintoxication via retro-orbital bleeding. Serum was separated by
centrifugation at 1665xg for 16 min, stored at −20 °C,
and shipped to Sandia National Laboratories for analysis. All procedures
involving animals were reviewed and approved by the Institutional
Animal Care and Use Committee, University of Massachusetts Dartmouth
(2003).
3
Longitudinal Monitoring of Hepatocellular Carcinoma in Rats
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Each week for 3 weeks, the rats were anesthetized with 2% isofluorane and 3 L/min oxygen and monitored via abdominal ultrasound. Mindray M7 ultrasound machine (Mindray North America, Mahwah, NJ, USA) with a Mindray L14-6a Linear Ultrasound Transducer (Mindray North America, Mahwah, NJ, USA). Ultrasound jelly was placed liberally on the rats’ abdomens and gently imaged using the ultrasound transducer. At 3-week post-implantation, both the 2.5 million and 5 million cell groups were also imaged with 1.5 T Aera MRI System (Siemens, Munich, Germany) and small 4-channel flex coil (TIM Systems, Siemens Medical Solutions, Erlangen, Germany). T2-weighted images were collected for monitoring the size of HCC. MR scans were performed in both coronal and axial orientations using a gradient-echo sequence. Tumor volume (V) was calculated as V = a2b/2 (where a is width, b is length, and a ≤ b). For comparison purposes, the tumor volume was normalized by its initial volume as V/V0 (V0 was the volume of the tumor at the time that catheterization). Prior to MRI scans, the animals were anesthetized with ketamine (Ketaset, Fort Dodge Animal Health, Fort Dodge, IA, USA) at 80 mg/kg and xylazine (Isothesia, Abbot Laboratories, Chicago, IL, USA) at 4 mg/kg via intraperitoneal injection.
4
Sinclair Swine Melanoma Model
5
High-Resolution Aortic Imaging in Mice
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The Vevo 770 High-Resolution Imaging system (Visualsonics, Toronto, Canada) with a RMV 707B transducer was used for all direct aortic examinations as described earlier [24 (link),26 (link),27 (link),30 (link)]. Briefly, each mouse was anesthetized with inhalation of 1.5% isoflurane (Isothesia, Abbot Laboratories, Chicago, USA) with oxygen and placed supine on a thermostatically heated platform to maintain a constant body temperature. All legs were taped to ECG electrodes for cardiac function monitoring. Abdominal hair was removed using a chemical hair remover (Church & Dwight Co., Inc., NJ, USA), pre-warmed US gel (Medline Industries, Inc., Mundelein, USA) was spread over the skin as a coupling medium for the transducer. Image acquisition was started on B-mode; two levels of the vessel were visualized longitudinally: thoracic region and renal region. Thereafter, a short-axis view was taken to visualize the same arterial site in a cross-sectional view immediately. For each level, individual frames and cine loops (300 frames) were acquired and recorded at distances of 1 mm throughout the length of the aorta. Measurements and data analysis was performed off line using the customized version of Vevo 770 Analytical Software from both the longitudinal and transverse images.
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