About 1 μg of total peptides was analyzed on a Waters M-Class UPLC using a 25-cm Ionopticks Aurora column coupled to a benchtop Thermo Fisher Scientific Orbitrap Q Exactive HF mass spectrometer. Peptides were separated at a flow rate of 400 nl/min with a 160-min gradient, including sample loading and column equilibration times. MS1 spectra were measured with a resolution of 120,000, an AGC target of 3 × 106, and a mass range from 300 to 1800 mass/charge ratio (m/z). Up to 12 MS2 spectra per duty cycle were triggered at a resolution of 60,000, an AGC target of 1 × 105, an isolation window of 0.8 m/z, a normalized collision energy of 28, a scan range of 200 to 2000 m/z, and a fixed first mass of 110 m/z.
Orbitrap q exactive hf mass
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The Orbitrap Q Exactive HF mass spectrometer is a high-performance analytical instrument designed for advanced proteomics and metabolomics applications. It combines a quadrupole mass analyzer with a high-resolution Orbitrap mass analyzer to provide accurate mass measurements and tandem mass spectrometry (MS/MS) capabilities.
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Lab products found in correlation
2 protocols using orbitrap q exactive hf mass
1
Peptide Separation and Identification via UPLC-MS/MS
2
Metabolomic analysis of SYQ roots by UHPLC-Orbitrap MS
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The roots of SYQ were grounded in liquid nitrogen and resuspended in 80% methanol. The suspensions were centrifuged at 12,000 rpm for 10 min at 4°C. Afterwards, the supernatant was diluted and transferred to a new tube. The sample extracts were analyzed by ultra-high performance liquid chromatography (UHPLC) (Thermo Fisher, Germany) coupled with an Orbitrap Q Exactive™ HF mass spectrometer (Thermo Fisher, Germany). Hypesil Gold column (100 × 2.1 mm, 1.9 μm) was used for the separation of compounds in extracts. The eluents in positive polarity mode were eluent A (0.1% formic acid in water) and eluent B (methanol), while eluents in negative polarity mode were eluent A (5 mM ammonium acetate, pH 9.0) and eluent B (methanol). The detailed parameters were set according to Li et al. (2023) (link). The mass fragmentations were blasted in the HMDB,4 METLIN5 and KEGG6 databases. SIMCA-P V12.0.0 Demo (Umetric, Umea, Sweden) was used for principal components analysis (PCA). Cluster analysis was conducted by R.
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