Percp anti mouse cd11c

Manufactured by BioLegend

Sourced in United States

PerCP anti-mouse CD11c is a fluorescently-labeled antibody that binds to the CD11c antigen expressed on the surface of mouse dendritic cells. It can be used to identify and quantify dendritic cells in flow cytometry applications.

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3 protocols using percp anti mouse cd11c

Comprehensive Immune Profiling of Murine Tumors

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Carprofen (Rimadyl^® Injectable, 50 mg/mL) was purchased from Patterson Companies (Saint Paul, MN, USA). Pacific Blue™ anti-mouse CD3, FITC anti-mouse CD4, PerCP anti-mouse CD8a, PE anti-mouse/human CD11b, APC/Cy7 anti-mouse Ly-6G/Ly-6C (Gr-1), PE/Cy7 anti-mouse CD62L, APC anti-mouse/human CD44, APC anti-mouse CD25, Biolegend PE anti-mouse/rat/human FOXP3, PerCP anti-mouse CD11c, PE/Cy7 anti-mouse CD86, FITC anti-mouse I-A/I-E, and APC anti-mouse CD40 were purchased from Biolegend (San Diego, CA, USA). Mouse tumor dissociation kit (No. 130-096-730) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).

Guo S., Burcus N.I., Hornef J., Jing Y., Jiang C., Heller R, & Beebe S.J. (2018). Nano-Pulse Stimulation for the Treatment of Pancreatic Cancer and the Changes in Immune Profile. Cancers, 10(7), 217.

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Enhancing CD8+ DC Presentation

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Mice were subcutaneously injected with 5 mg CytC (Sigma-Aldrich) on each side of the tail base every 12 h for 4 injections in total. 24 h after the last injection, cell suspensions from the spleen and draining LNs were prepared to enrich DCs as described above. The enriched DCs were then stained with PerCP anti-mouse CD11c (clone: N418, BioLegend) and FITC anti-mouse CD8α (clone: 53–6.7, BioLegend) to determine the fraction of CD8+ DCs by flow cytometry. To detect the pOVA epitopes presentation in mice that were treated with CytC, iTEP-sMMP-pOVA was injected subcutaneously at one hour after the last injection of CytC. 24 h later, DCs from the mouse spleen and draining LNs were collected to do B3Z activation assay as described above.

Wang P., Dong S., Zhao P., He X, & Chen M. (2018). Direct loading of CTL epitopes onto MHC class I complexes on dendritic cell surface in vivo. Biomaterials, 182, 92-103.

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Bone Marrow-Derived Cell Activation

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To verify the maturation and activation status of BMDCs upon exposure to LMs and the three inhibitors, flow cytometry were carried out. Briefly, BMDCs were stained with a combination of antibodies (AF-700 anti-mouse MHCII [Biolegend, USA], PE-anti mouse CD86 [Biolegend, USA] and PerCP anti-mouse CD11c [Biolegend, USA]) on ice for 20 min. BMDCs were washed with staining buffer (3% fetal bovine serum in 1X PBS) to block any nonspecific antibody binding, resuspended in PBS in the dark on ice for 30 min before acquisition by Flow Cytometer FACS CANTO II. BMDCs were acquired with FACS Diva™ software from BD Biosciences (New Jersey, USA) and analyzed by FlowJo ® software from BD Biosciences (New Jersey, USA).

Mat Luwi N.E., Kadir R., Mohamud R., A Garcia-Santana M.L., Acevedo R., Sarmiento M.E., Norazmi M.N, & Acosta A. (2020). Liposomes derived from Mycobacterium smegmatis promote immune activation of mice bone marrow-derived dendritic cells. International journal of mycobacteriology, 9(3).

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