Potassium oxonate (PO), allopurinol, and oxonic acid (potassium salt) were purchased from Sigma-Aldrich Chemie GmbH (Schnelldorf, Germany). L. plantarum powder (TCI227) was prepared by TCI Co. Ltd. A uric acid assay kit was purchased from Abcam (Cambridge, UK). Sodium chloride (NaCl) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Formalin (HCHO) was purchased from Choneye Co., Ltd. (New Taipei City, Taiwan).
Oxonic acid
Manufactured by Merck Group
Sourced in United States
Oxonic acid is a chemical compound used in laboratory settings. It serves as a precursor for the production of various organic compounds. The core function of oxonic acid is to facilitate chemical reactions and synthesis in controlled laboratory environments.
Automatically generated - may contain errors
Lab products found in correlation
Uric acid, by Merck Group (2 mentions)
Sodium chloride (nacl), by Fujifilm (1 mentions)
Potassium oxonate, by Merck Group (1 mentions)
Allopurinol, by Merck Group (1 mentions)
Uric acid assay kit, by Abcam (1 mentions)
Griess reagent, by Merck Group (1 mentions)
2 7 dichlorofluorescin diacetate dcfh da, by Thermo Fisher Scientific (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Mip 2, by R&D Systems (1 mentions)
Mouse antibody for α tubulin, by Merck Group (1 mentions)
Adma elisa kit, by Enzo Life Sciences (1 mentions)
Enzychrom nadp nadph assay kit ecnp 100, by BioAssay Systems (1 mentions)
Hydroethidine dhe, by Thermo Fisher Scientific (1 mentions)
Mcp 1, by R&D Systems (1 mentions)
Uricase, by Merck Group (1 mentions)
Media for cell culture, by Thermo Fisher Scientific (1 mentions)
Ab2413, by Abcam (1 mentions)
Ab34710, by Abcam (1 mentions)
Ab49311, by Abcam (1 mentions)
Anti β actin, by Bioworld Technology (1 mentions)
4 protocols using oxonic acid
1
Uric Acid Regulation by L. plantarum
2
Oxidative Stress and Inflammatory Mediators in Vascular Disease
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Uric acid, oxonic acid, ADMA, Griess reagent, N‐acetylcysteine (NAC), apocynin (APO), and mouse antibody for α-tubulin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Rabbit antibodies for DDAH-1, DDAH-2, protein arginine methyltransferases 1 (PRMT1), and cationic amino acid transporter-1 (CAT-1) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit antibodies for intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were purchased from Cell Signaling Technology (Beverly, MA, USA). Our ADMA ELISA kit was obtained from Enzo Life Sciences (Farmingdale, NY, USA). Our EnzyChrom NADP+/NADPH assay kit (ECNP-100) was obtained from BioAssay Systems (Hayward, CA, USA). Hydroethidine (DHE) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were obtained from Molecular Probes (Eugene, OR, USA). Our ELISA kits for tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-6, monocyte chemoattractant protein 1 (MCP-1), and macrophage inflammatory protein 2 (MIP-2) were obtained from R&D Systems (Minneapolis, MN, USA).
3
Comprehensive Reagent Procurement Protocol
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Most reagents, buffers, substrates, uric acid, uricase, and oxonic acid were purchased from Sigma Chemical Company (St Louis, MO, USA). Media for cell culture were obtained from Gibco/BRL (Bethesda, MD, USA). Hematoxylin Nuclear Counterstain (H-3401) was purchased from Vector Laboratories (Burlingame, CA, USA) for use in hematoxylin and eosin tissue staining.
4
Hepatic Uricase Inhibition and Fibrosis
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Oxonic acid (OA; a hepatic uricase inhibitor) and UA were purchased from Sigma (St. Louis, MO, USA). Anti-β-actin (1:1000, BS1002) and anti-pSRF (1:1000, BS4177) antibodies were obtained from Bioworld Technology (Louis Park, MN, USA); anti-SRF antibody (1:500, sc-335) and anti-slug (1:500, sc-166902) antibodies were obtained from Santa Cruz (Santa Cruz, CA, USA); and anti-collagen I (1:1000, ab34710), anti-E-cadherin (1:1000, ab76055), anti-fibronectin (FN) (1:1000, ab2413), anti-α-smooth muscle actin (α-SMA) (1:1000, ab5694), anti-Fibroblast specific protein-1 (FSP-1) (1:1000, ab27957) and anti-MRTF-A (1:1000, ab49311) antibodies were obtained from Abcam (Cambridge, MA, USA). CCG-1423 was obtained from Cayman Chemical (Ann Arbor, MI, USA).
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