Surface morphology of the prepared nanoplexes was assessed using TEM. A drop (10 μl) of aqueous solution of the nanoplexes (N/P 20) was added for 30 sec on a carbon coated, glow discharged 400-mesh TEM copper grid that was pre-coated with a Formvar®. Whatman® filter paper was then used to remove any excess liquid and the grid was air dried. Negative staining was then performed by adding a drop of freshly prepared 1% w/v phosphotungstic acid solution for 30 sec followed by removing the excess stain as described before. TEM images were captured using JEOL JEM-1230 transmission electron microscope equipped with a Gatan UltraScan 1000 2k × 2k CCD acquisition system. Captured images were processed using ImageJ (Image Processing and Analysis in Java, Version 1.47).
Ultrascan 1000 2k 2k ccd
Manufactured by Ametek
The UltraScan 1000 is a 2k × 2k CCD (Charged Coupled Device) for laboratory equipment. It is designed to capture high-resolution images for scientific and research applications.
Automatically generated - may contain errors
2 protocols using ultrascan 1000 2k 2k ccd
1
Nanocomplex Surface Morphology Analysis
2
Transmission Electron Microscopy of Plant Leaves
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Transmission electron microscopy was conducted as described previously in Caplan et al. (2015) (link). Leaf excisions were fixed with 2% paraformaldehyde and 2% gluaraldehyde in PHEM (60 mM PIPES, 25 mM HEPES, 10 mM EGTA, and 2 mM MgCl2, pH 6.9) buffer for 45 min) overnight at 4°C. Samples were washed with 0.1 M sodium cacodylate buffer (pH 7.4), postfixed with 1% osmium tetroxide in the same buffer for 2 hr, and then washed with buffer and water. Samples were dehydrated in an acetone series (25%, 50%, 75%, 95%, and twice in anhydrous 100% acetone; 30 min each step) and infiltrated with Quetol 651-NSA resin. Ultrathin serial sections were cut on a Reichert-Jung Ultracut E ultramicrotome and collected onto a film of 0.5% formvar using 2 × 1 single slot grids. Sections were post-stained with methanolic uranyl acetate and Reynolds’ lead citrate and examined with a Zeiss Libra 120 TEM operating at 120kV. Images were acquired with a Gatan Ultrascan 1000 2k × 2 k CCD.
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