Cells were lysed in NP40 buffer (Biosource, Invitrogen, Paisley, UK). Samples containing equal amounts of protein were electrophoresed under reducing conditions in 8–10% SDS-PAGE gels. Protein was electroblotted to polyvinylidene difluoride (PVDF) membranes (Amersham Biosciences, Buckinghamshire, UK). Blots were probed against primary antibodies of interest and horseradish peroxidase-conjugated anti-rat or anti-mouse (Dako) were used as secondary antibodies. Bound antibodies were detected with the enhanced chemiluminescence Western blotting detection kit system (Amersham). Blots were probed for HSC70 (Santa Cruz Biotechnology) as a loading control.
Enhanced chemiluminescence western blotting detection kit system
Manufactured by Cytiva
The Enhanced chemiluminescence Western blotting detection kit system is a laboratory equipment product that facilitates the detection and visualization of proteins in Western blot analysis. It provides a reliable and sensitive method for the detection of target proteins in complex biological samples.
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2 protocols using enhanced chemiluminescence western blotting detection kit system
1
Western Blot Analysis of Protein Expression
2
Western Blot Analysis of MCT1 Protein
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Cells were lysed in NP40 buffer (Biosource, Invitrogen, Paisley, UK). Samples containing equal amounts of protein were electrophoresed under reducing conditions in 8–10% SDS-PAGE gels. Protein was electroblotted to PVDF membranes (Amersham Biosciences, Buckinghamshire, UK). Blots were probed with antibodies against MCT1 (Santa Cruz Biotechnology). Horseradish peroxidase-conjugated anti-rat or anti-mouse (Dako) was used as secondary antibodies. Bound antibodies were detected with the enhanced chemiluminescence western blotting detection kit system (Amersham). Blots were probed for Total FAK (Millipore) as a loading control.
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