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Snrnp40 sab2701506 and srsf2 sc35 clone sc 35

Manufactured by Merck Group

SNRNP40 (SAB2701506) is a laboratory reagent used for research purposes. It is a recombinant protein that can be used for applications such as immunoprecipitation and Western blotting. SRSF2/SC35 (clone SC-35) is an antibody that can be used to detect the SRSF2 protein, which is involved in RNA splicing. This antibody can be used for techniques like immunofluorescence and immunohistochemistry.

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2 protocols using snrnp40 sab2701506 and srsf2 sc35 clone sc 35

1

Comprehensive Chromatin Immunoprecipitation and Protein Interaction Mapping

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MNase and all chemicals were purchased from Sigma-Aldrich unless otherwise specified. DNA modifying enzymes were from Roche Applied Sciences. Pladienolide B was from Santa Cruz Biotechnology, the ATR inhibitor VE821 from TINIB-Tools, and the ATM inhibitor KU55933 and DNA-PK inhibitor NU7441 from R&D Systems. Antibodies used were against: PRP8 (H300), XPA/p62 (FL-273), p89/XPB (S-19) and β-Tubulin from Santa Cruz Biotechnology; SNRPC/U1C (NBP1-96048), NHP2L1 (NBP1-32732), SF3a1 (NB100-79847), SF3b2 (NV100-79843), RNaseH1 (NBP2-20171), and RNaseH2A (NBP1-76981) from Novus Biologicals; SNRNP40 (SAB2701506) and SRSF2/SC35 (clone SC-35) from Sigma; SNRPA/U1A (3F9-1F7) from ABGENT; PRPF3 (ab187535), RNPII CTD (phospho-S2) (ab5095), RNAPII (ab5095), PCNA (PC-10), Ki67 (ab833) from abcam®; CPD (TDM-2) from MBL International; GFP (11 814 460 001) from Roche; H2A (07-146) from Millipore Corp.; phosphor-ATM(1981)(05-740) from Upstate Biotechnology, Phospho-CHK2(Thr68) (2661) from Cell Signaling. Anti-XPC (rabbit-polyclonal ab) was in-house developed. Odyssey-compatible IRDye680- and IRDye800–conjugated secondary antibodies were from LI-COR. Secondary antibodies conjugated to Alexa Fluorochromes-488, -568, -594 and-647 were from Invitrogen. GFP-tagged proteins were immunoprecipitated with GFP-Trap® beads (ChromoTek).
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2

Comprehensive Chromatin Immunoprecipitation and Protein Interaction Mapping

Check if the same lab product or an alternative is used in the 5 most similar protocols
MNase and all chemicals were purchased from Sigma-Aldrich unless otherwise specified. DNA modifying enzymes were from Roche Applied Sciences. Pladienolide B was from Santa Cruz Biotechnology, the ATR inhibitor VE821 from TINIB-Tools, and the ATM inhibitor KU55933 and DNA-PK inhibitor NU7441 from R&D Systems. Antibodies used were against: PRP8 (H300), XPA/p62 (FL-273), p89/XPB (S-19) and β-Tubulin from Santa Cruz Biotechnology; SNRPC/U1C (NBP1-96048), NHP2L1 (NBP1-32732), SF3a1 (NB100-79847), SF3b2 (NV100-79843), RNaseH1 (NBP2-20171), and RNaseH2A (NBP1-76981) from Novus Biologicals; SNRNP40 (SAB2701506) and SRSF2/SC35 (clone SC-35) from Sigma; SNRPA/U1A (3F9-1F7) from ABGENT; PRPF3 (ab187535), RNPII CTD (phospho-S2) (ab5095), RNAPII (ab5095), PCNA (PC-10), Ki67 (ab833) from abcam®; CPD (TDM-2) from MBL International; GFP (11 814 460 001) from Roche; H2A (07-146) from Millipore Corp.; phosphor-ATM(1981)(05-740) from Upstate Biotechnology, Phospho-CHK2(Thr68) (2661) from Cell Signaling. Anti-XPC (rabbit-polyclonal ab) was in-house developed. Odyssey-compatible IRDye680- and IRDye800–conjugated secondary antibodies were from LI-COR. Secondary antibodies conjugated to Alexa Fluorochromes-488, -568, -594 and-647 were from Invitrogen. GFP-tagged proteins were immunoprecipitated with GFP-Trap® beads (ChromoTek).
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