Dactinomycin

Manufactured by Selleck Chemicals

Sourced in China

Dactinomycin is a laboratory compound used in various research applications. It is a polypeptide antibiotic with a core function of inhibiting RNA synthesis. This compound is commonly used in biochemical and cellular studies.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Genistein, by Cayman Chemical (1 mentions) Mitotempo, by Cayman Chemical (1 mentions) Trichostatin a, by Cayman Chemical (1 mentions) Melatonin, by Cayman Chemical (1 mentions) γ tocotrienol, by Cayman Chemical (1 mentions) Auranofin, by Cayman Chemical (1 mentions) Calcein am, by Thermo Fisher Scientific (1 mentions) Cycloheximide (chx), by Selleck Chemicals (1 mentions) Du145, by Thermo Fisher Scientific (1 mentions) Lncap, by Thermo Fisher Scientific (1 mentions) Gefitinib, by Selleck Chemicals (1 mentions) Gibco dmem medium, by Thermo Fisher Scientific (1 mentions) Gibco rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)

3 protocols using dactinomycin

Diverse Chemical Compounds Protocol

Cited 5 times

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Dactinomycin and piplartine were purchased from Selleck Chemicals (Houston TX); Melatonin^{22 (link)}, Trichostatin A^{26 (link)}, γ-Tocotrienol^{25 (link)}, Auranofin^{18 (link)}, Genistein^{27 (link)}, and Mito-Tempo^{20 (link)} from Cayman chemicals (Ann Arbor, MI); GC1149^{19 (link)} from Galera Therapeutics (Malvern, PA); Recilisib sodium (also named as Ex-Rad)^{17 (link)} from MedKoo Biosciences (Morrisville, NC; V028–5832 (also named as compound C)^{21 (link)} and Y600–0815(also named as PKUMDL-LC-101)^{23 (link)} from Enamine, (Monmouth Junction, NJ). The stock solutions of all these chemicals at 10 mM in DMSO were prepared, unless specified otherwise.
Cell-permeable Hoechst 33342 dye, and Calcein-AM were from Thermo Fisher Scientific (Waltham, MA). 1 mM and 2 mM (1000x) stock solutions were prepared according to the vender’s instructions, respectively.

Wen K.K., Roy S., Grumbach I.M, & Wu M. (2021). A “failed” assay development for the discovery of rescuing small molecules from the radiation damage. SLAS discovery : advancing life sciences R & D, 26(10), 1315-1325.

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Stability of FOXM1 mRNA in C918 cells

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C918 cells were exposed to 2 μg/mL of dactinomycin (Selleck, Shanghai, China) to block transcription for 0, 3, 6, and 12 hours. The cells were harvested, and the stability of FOXM1 mRNA was analyzed using qRT-PCR assay.

Hao L., Yin J., Yang H., Li C., Zhu L., Liu L, & Zhong J. (2021). ALKBH5-mediated m6A demethylation of FOXM1 mRNA promotes progression of uveal melanoma. Aging (Albany NY), 13(3), 4045-4062.

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Cell Culture and Drug Treatments

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All the cell lines were purchased from the Stem Cell Bank, Chinese Academy of Sciences in Shanghai, China, and verified by the China Centre for Type Culture Collection in Wuhan, China. PCa cell lines (PC-3, DU-145, LNCap, and 22RV1) were cultured in Gibco RPMI-1640 medium (Thermo Fisher Scientific, USA). HEK293T cell line was cultured in Gibco DMEM medium (Thermo Fisher Scientific, USA). All the cell cultures were supplemented with 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, USA) and cultured at 37 °C in a humidified incubator containing 5% CO₂. Gefitinib (S1025, Selleck), Cycloheximide (S7418, Selleck), Dactinomycin (S8964, Selleck), and EG01377 (HY-112151, MCE) were purchased from commercial sources indicated.

Zhang P., Chen L., Zhou F., He Z., Wang G, & Luo Y. (2023). NRP1 promotes prostate cancer progression via modulating EGFR-dependent AKT pathway activation. Cell Death & Disease, 14(2), 159.

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