Alexa Fluor 488 succinimidyl ester (‘Alexa488’), the 488 nm excitable lipid analogues 3,3′-dioctadecyloxa-carbocyanine perchlorate (‘DiO’; DiO-C18(3)), 3,3′-dilinoleyloxa-carbocyanine perchlorate (‘fast-DiO’; DiO∆9,12-C18(3) solid), the 543 nm excitable lipid analogue 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindo-carbocyanine perchlorate (‘DiI’; DiI-C18(3)), and the 633 nm excitable lipid analogue 1,1′-dioctadecyl-3,3,3′,3′ tetramethylindo-dicarbocyanine, 4-chlorobenzenesulfonate salt (‘DiD’; DiI-C18(5) solid) were purchased from Thermo Fisher Scientific (Darmstadt, Germany). 1,2-dioleoyl-sn-glycero-3-phosphocholine (dioleoylphosphatidylcholine; DOPC), brain sphingomyelin (BSM), and cholesterol (Chol) were purchased from Avanti Polar Lipids (Alabaster, AL, USA) and used without further purification. Ultra-low gelling temperature agarose (type IX-A), paraformaldehyde (PFA), dithiothreitol (DTT), and other chemicals were purchased from Sigma-Aldrich (Munich, Germany). Random labeling of interleukin-4 (IL-4) ligand with amino-reactive Alexa Fluor 647 was performed as described (Weidemann et al. 2011 (link)).
1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine 4 chlorobenzenesulfonate salt did
Manufactured by Thermo Fisher Scientific
Sourced in Germany, Japan, United States
1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine,4 Chlorobenzenesulfonate Salt (DiD) is a fluorescent lipophilic carbocyanine dye. It is used as a cell membrane labeling agent.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Glycerol, by Merck Group (2 mentions)
4 6 diamidino 2 phenylindole dapi stain, by Thermo Fisher Scientific (2 mentions)
Poly l lysine coated glass slides, by Avantor (2 mentions)
Naio4, by Merck Group (2 mentions)
Ir 780 iodide, by Merck Group (2 mentions)
Aldehyde quantification assay kit fluorometric, by Abcam (2 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate, by Thermo Fisher Scientific (1 mentions)
Cholesterol chol, by Avanti Polar Lipids (1 mentions)
3 3 dioctadecyloxacarbo cyanine perchlorate, by Thermo Fisher Scientific (1 mentions)
Calcein, by Dojindo Laboratories (1 mentions)
Cholesterol, by Tokyo Chemical Industry (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Thermo Fisher Scientific (1 mentions)
Nanobrook omi, by Brookhaven Instruments (1 mentions)
Iron 3 chloride, by Merck Group (1 mentions)
Magnesium sulfate, by Merck Group (1 mentions)
Dulbecco s phosphate buffered saline, by Merck Group (1 mentions)
Ammonium iron 2 sulfate hexahydrate, by Merck Group (1 mentions)
Copper 2 sulfate, by Merck Group (1 mentions)
Thioglycolic acid, by Merck Group (1 mentions)
6 protocols using 1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine 4 chlorobenzenesulfonate salt did
1
Fluorescent Lipid Analogues and Labeling
2
Lipid Components for Liposomes
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For lipid components, 1, 2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) and cholesterol were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan), 1, 5-dihexadecyl-N-succinyl-L-glutamate (DHSG) was purchased from Nippon Fine Chemical Co. Ltd. (Osaka, Japan), and 1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[monomethoxy poly (ethylene glycol) (5000)] (PEG-DSPE) was purchased from NOF Co. Ltd. (Tokyo, Japan). For fluorescent dyes, calcein was purchased from Dojindo Laboratories (Kumamoto, Japan), while 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD) was purchased from Thermo Fisher Scientific (Waltham, United States).
3
Preparation and Characterization of Influenza Virus Mutants
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Two Udorn mutants were prepared according to previous works: Udorn with an HA mutation for preferential α2,3-Sia binding (Ud23), and Udorn with an HA mutation for preferential α2,6-Sia binding (Ud26)20 (link),39 (link). Ud23 has two mutations in the receptor binding domain, L226Q and S228G, while Ud26 has a single mutation in the receptor binding domain, E190D20 (link). Udorn IAV was labeled with a lipophilic dye, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI; Invitrogen) while Ud23 and Ud26 IAV were labeled with a different lipophilic dye with a longer excitation and emission wavelength, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine 4-chlorobenzenesulfonate salt (DiD; Invitrogen). IAV sizes were determined (Figure S4 ) via dynamic light scattering (DLS) using the NanoBrook Omi (Brookhaven Instruments).
4
Characterizing Sunscreen Composition and Effects
5
Electrochemical Metal Ion Sensors
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Polyvinyl
chloride (PVC), bis ethylhexyl
sebacate (DOS), bathophenanthroline (BP), 8-hydroxyquinoline (8HQ),
bathocuproine (BC), tetrahydrofuran (THF), dichloromethane (DCM),
sodium tetrakis-[3,5-bis(trifluoromethyl)phenyl]borate (NaBARF; Selectophore),
sodium acetate, glacial acetic acid, Dulbecco’s phosphate-buffered
saline (PBS), ammonium iron(II) sulfate hexahydrate, iron(III) chloride,
cobalt(II) sulfate hydrate, copper(II) sulfate, nickel(II) sulfate,
magnesium sulfate, and thioglycolic acid were all purchased from Sigma-Aldrich
(St. Louis, MI, USA). 3,3′-Dioctadecyloxacarbocyanine perchlorate
(DiO; Invitrogen) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine,
4-chlorobenzenesulfonate salt (DiD; Invitrogen) were purchased from
Thermo Fisher Scientific (Waltham, MA, USA). Microdialysis hollow
fibers (13 kDa cutoff) were purchased from Spectrum Laboratories,
Repligen (Waltham, MA, USA). Ferrous sulfate supplement tablets were
purchased from Signature Care. Creek water was obtained from Clear
Creek in Golden, CO, USA on February 21, 2021.
chloride (PVC), bis ethylhexyl
sebacate (DOS), bathophenanthroline (BP), 8-hydroxyquinoline (8HQ),
bathocuproine (BC), tetrahydrofuran (THF), dichloromethane (DCM),
sodium tetrakis-[3,5-bis(trifluoromethyl)phenyl]borate (NaBARF; Selectophore),
sodium acetate, glacial acetic acid, Dulbecco’s phosphate-buffered
saline (PBS), ammonium iron(II) sulfate hexahydrate, iron(III) chloride,
cobalt(II) sulfate hydrate, copper(II) sulfate, nickel(II) sulfate,
magnesium sulfate, and thioglycolic acid were all purchased from Sigma-Aldrich
(St. Louis, MI, USA). 3,3′-Dioctadecyloxacarbocyanine perchlorate
(DiO; Invitrogen) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine,
4-chlorobenzenesulfonate salt (DiD; Invitrogen) were purchased from
Thermo Fisher Scientific (Waltham, MA, USA). Microdialysis hollow
fibers (13 kDa cutoff) were purchased from Spectrum Laboratories,
Repligen (Waltham, MA, USA). Ferrous sulfate supplement tablets were
purchased from Signature Care. Creek water was obtained from Clear
Creek in Golden, CO, USA on February 21, 2021.
6
Characterizing Sunscreen Composition and Effects
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IR-780 iodide, glycerol, NaIO4 and Bovine serum albumin (BSA) were obtained from the Sigma-Aldrich. The 1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine,4 Chlorobenzenesulfonate Salt (DiD) and 4,6-diamidino-2 phenylindole (DAPI) stain were ordered from Invitrogen. Aldehyde Quantification Assay Kit (Fluorometric) was from Abcam. Poly -L -lysine coated glass slides were obtained from VWR International Inc..Walgreens sunscreen lotion (SPF 30,, Avobenzone 3.0%, Homosalate 10.0%, Octisalate 5.0%, Octocrylene 10%) was purchased from Walgreens. Sunscreen oil (SPF 30, Avobenzone 3.0%, Homosalate 10.0%, Octisalate 5.0%, Octocrylene 7%) was from L'Oréal Paris.
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