Primesoript rt master mix

Manufactured by Takara Bio

Primesoript RT Master Mix is a ready-to-use solution for reverse transcription of RNA into cDNA. It contains all the necessary components for the reverse transcription reaction, including reverse transcriptase, reaction buffer, and dNTPs.

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Lab products found in correlation

Sybr qpcr master mix, by Takara Bio (2 mentions)

Close spelling variants of this product

RT Master Mix

2 protocols using primesoript rt master mix

Quantifying Gene Expression Changes in TAD Reorganization

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Timing: 1 day

RT-qPCR is performed to check the expression change of related genes in response to TAD reorganization

33.

RNA extraction and reverse transcriptiona.

Total RNA was extracted from cell pellets using RNAzol reagent (MRC) and cDNA was synthesized using Primesoript RT Master Mix (Takara).

34.

Real time qPCRa.

qPCR was performed using SYBR qPCR Master Mix on LightCycler 480 II system.

35.

Data analysisa.

The fold change (FC) of experimental group versus control group was calculated. △Ct was calculated as △Ct = Ct (test gene) – Ct (Ref. gene). △△Ct was calculated as △△Ct = △Ct (experimental group) – △Ct (control group). The FC of a test gene in experimental group versus control group was calculated as FC = 2ˆ(-△△Ct). Each gene tested in triplicates in every independent experiment, and all experiments were triplicated.

Wang J., Ma Q., Fang P., Tian Q., Yu H., Sun J, & Ding J. (2021). Manipulation of TAD reorganization by chemical-dependent genome linking. STAR Protocols, 2(3), 100799.

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Evaluating Pluripotency Gene Expression

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Timing: 1 day

This step is performed to check the expressions of pluripotency genes in response to each perturbation of OCT4.

55.

RNA extraction and reverse transcriptiona.

Total RNA was extracted from cell pellets using RNAzol reagent (MRC) and cDNA was synthesized using Primesoript RT Master Mix (Takara).

56.

qPCRa.

qPCR was performed using SYBR qPCR Master Mix on LightCycler 480 II system.

57.

Data analysisa.

The fold change (FC) of experimental group versus control group was calculated. Ct was calculated with Ct = Ct (test gene) - Ct (Ref. gene). Ct was calculated with Ct = Ct (mutant or rescue group) – Ct (wide type group).

The FC of a test gene in experimental group versus control group was calculated with FC = 2ˆ(-Ct).

Each gene tested in triplicates in every independent experiment, and all experiments were triplicated.

Ma Q., Wang J., Yu H., Sun J., Sun J., Chen J., Yu Y., Fang P., Tian Q, & Ding J. (2021). Protocol to alter a protein’s phase separation capacity to control cell fate transitions. STAR Protocols, 2(4), 100887.

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