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Fluorescein azide

Manufactured by Lumiprobe
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Fluorescein-azide is a fluorescent dye compound that can be used in various biological and chemical applications. It consists of a fluorescein moiety and an azide functional group. The fluorescent properties of fluorescein allow for detection and visualization, while the azide group enables conjugation to other molecules.

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Lab products found in correlation

Alexa fluor 488 azide, by Thermo Fisher Scientific (2 mentions) Sulforhodamine101 azide, by Vector Laboratories (2 mentions) Cy5.5 azide, by AAT Bioquest (2 mentions) Alexa fluor 647 azide, by Thermo Fisher Scientific (2 mentions) Tamra azide, by Vector Laboratories (2 mentions)

3 protocols using fluorescein azide

1

Fluorescent Azido-Dye Labeling Protocol

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Fluorescein-azide was purchased from Lumiprobe. AlexaFluor488-azide and AlexaFluor647-azide were purchased from Life Technologies. TAMRA-azide and Sulforhodamine101-azide were purchased from Click Chemistry Tools. Cy5.5 azide was purchased from AAT Bioquest.
Ngo J.T., Adams S.R., Deerinck T.J., Boassa D., Rodriguez-Rivera F., Palida S.F., Bertozzi C.R., Ellisman M.H, & Tsien R.Y. (2016). Click-electron microscopy for imaging metabolically tagged non-protein biomolecules. Nature chemical biology, 12(6), 459-465.
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2

Fluorescent Azido-Dye Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescein-azide was purchased from Lumiprobe. AlexaFluor488-azide and AlexaFluor647-azide were purchased from Life Technologies. TAMRA-azide and Sulforhodamine101-azide were purchased from Click Chemistry Tools. Cy5.5 azide was purchased from AAT Bioquest.
Ngo J.T., Adams S.R., Deerinck T.J., Boassa D., Rodriguez-Rivera F., Palida S.F., Bertozzi C.R., Ellisman M.H, & Tsien R.Y. (2016). Click-electron microscopy for imaging metabolically tagged non-protein biomolecules. Nature chemical biology, 12(6), 459-465.
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3

Fluorescent PEG Conjugation Protocol

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To a reaction vial equipped with a stir bar was added 8 arm PEG DBCO (Mn = 20 kg mol−1, 2.4 g, 0.12 mmol) and PBS (19 mL). Fluorescein-azide (Lumiprobe (Cockeysville, MD), FAM azide, 6-isomer) stock solution was made in DMSO (10 mg/mL). FAM azide (1 mL, 0.015 mmol) was added to the reaction vial and stirred for 1 h at room temperature in the dark. The functionalized PEG DBCO was then dialyzed extensively against water (MWCO 8 kDa) protected from light, then lyophilized.
Macdougall L.J., Hoffman T.E., Kirkpatrick B.E., Fairbanks B.D., Bowman C.N., Spencer S.L, & Anseth K.S. (2022). Intracellular Crowding by Bio-Orthogonal Hydrogel Formation Induces Reversible Molecular Stasis. Advanced materials (Deerfield Beach, Fla.), 34(31), e2202882.
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