Catalog td 80396

Male Sprague-Dawley albino rats (Charles River Laboratories, Wilmington, MA), weighing 80–100 grams and 250–350 g at the time of the first and second surgeries, respectively, were used in the experiments. Animals were housed 2 per cage and kept on a 12/12-h dark/light cycle with food and water available ad libitum. All animals used in the study were maintained in accordance with the guidelines of the National Institutes of Health Animal Care and the animal research conducted to perform this study was approved by the NIDA IRP Animal Care and Use Committee (protocol #: 15-BNRB-73). The rats were divided into two groups: the control group was fed with a diet containing an essential amount of iron (48 ppm iron, Catalog TD.80394, Harlan-Teklad, Madison, WI) and the BID group was fed with a diet containing a low iron concentration (4 ppm iron, Catalog TD.80396, Harlan-Teklad). The other contents of the diet were the same. Diets were started immediately after weaning and continued for 21 days, when microdialysis experiments were performed.

Male Sprague-Dawley albino rats (Charles River Laboratories, Wilmington, MA), weighing 100–150 grams and 280–360 g at the time of the first and second surgeries, respectively, were used in the experiments. Animals were housed 2 per cage and kept on a 12/12-h dark/light cycle with food and water available ad libitum. All animals used in the study were maintained in accordance with the guidelines of the National Institutes of Health Animal Care and the animal research conducted to perform this study was approved by the NIDA IRP Animal Care and Use Committee (protocol #: 15-BNRB-73). The rats were divided into two groups: the control group was fed with a diet containing an essential amount of iron (48 ppm iron, Catalog TD.80394, Harlan-Teklad, Madison, WI) and the BID group was fed with a diet containing a low iron concentration (4 ppm iron, Catalog TD.80396, Harlan-Teklad). The other contents of the diet were the same. Diets were started immediately after weaning, at 21 days of age, and continued for four weeks, when microdialysis experiments were performed.