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The MKN74 is a laboratory instrument designed for the analysis and processing of biological samples. It is a compact and versatile device that can be used for a variety of applications in research and clinical settings. The core function of the MKN74 is to provide accurate and reliable data processing capabilities for researchers and scientists working in the field of health sciences.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions) Kato 3, by Health Science Research Resources Bank (2 mentions) Mkn45, by Health Science Research Resources Bank (1 mentions) Nugc 4, by Health Science Research Resources Bank (1 mentions) Az521, by RIKEN BioResource Center (1 mentions) Ke 97, by RIKEN BioResource Center (1 mentions) Ecc12, by RIKEN BioResource Center (1 mentions) Nci n87, by American Type Culture Collection (1 mentions) Snu 5, by American Type Culture Collection (1 mentions) Kato 3, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Recombinant human interferon ifn γ, by Thermo Fisher Scientific (1 mentions) Ocum 1, by Health Science Research Resources Bank (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Mkn74, by Thermo Fisher Scientific (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

3 protocols using mkn74

1

Establishment of Gastric Cancer Cell Lines and Fibroblasts

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Human gastric cancer cell lines 58As9, HSC-59, HSC-44PE, and 44As3 were described previously [16] (link), [17] (link), and MKN1, MKN7, and MKN74 were obtained from the Health Science Research Resources Bank. 44As3 cells stably expressing tdTomato were generated by retroviral transduction. These cells were maintained in RPMI 1640 medium (Invitrogen) supplemented with 10% FBS and antibiotics at 37°C in a humidified atmosphere containing 5% CO2. The orthotopic fibroblast cell lines, CaF37 and CaF38 were established from the tumoral gastric wall of SGC patients who had undergone gastrectomy. The primary gastric tumor was excised under aseptic conditions and minced with forceps and scissors. The tumor pieces were cultivated in DMEM (Nikken) with 10% FBS. After approximately 2 weeks, fibroblasts were collected and transferred to another culture dish. Serial passages were carried out every 4–7 days. The fibroblasts used were 4–10th passage of culture. This study was approved by the Osaka City University ethics committee and written informed consent was obtained from the patients prior to the study.
Yamaguchi H., Yoshida N., Takanashi M., Ito Y., Fukami K., Yanagihara K., Yashiro M, & Sakai R. (2014). Stromal Fibroblasts Mediate Extracellular Matrix Remodeling and Invasion of Scirrhous Gastric Carcinoma Cells. PLoS ONE, 9(1), e85485.
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2

Gastric Cancer Cell Line Cultivation

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The human gastric cancer cell lines used, that is, HSC-39, HSC-43, HSC-59, HSC-60, HSC-64, HSC-44PE, 58As9, 58As1, 44As3 and 44As3Luc, have been described previously.31 (link)–34 (link) MKN1, MKN7, MKN74, NUGC-4, KATO-III, MKN45 and IM95 were obtained from the Health Science Research Resources Bank. AGS, NCI-N87 and SNU-5 were obtained from the American Type Culture Collection (ATCC). GCIY, ECC12, AZ521 and KE-97 were provided by the RIKEN Bio-Resource Center through the National Bio-Resource Project of the MEXT, Japan. These cells were maintained in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS, 10 units/mL of penicillin and 10 μg/mL of streptomycin at 37°C in a humidified atmosphere containing 5% CO2.
Yamaguchi H., Takanashi M., Yoshida N., Ito Y., Kamata R., Fukami K., Yanagihara K, & Sakai R. (2014). Saracatinib impairs the peritoneal dissemination of diffuse-type gastric carcinoma cells resistant to Met and fibroblast growth factor receptor inhibitors. Cancer Science, 105(5), 528-536.
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3

Culturing Human Gastric Cancer Cells

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The human GC cell lines MKN1, MKN74, KATO III, OCUM-1 were obtained from the Health Science Research Resources Bank. MKN1, MKN74, and OCUM-1 cells were cultured in Dulbecco's modified Eagle's medium (Invitrogen; Thermo Fisher Scientific, Inc.) and KATO III cells were cultured in RPMI-1640 medium (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal calf serum (Invitrogen; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.) placed at 37°C in a humidified incubator containing 5% CO2. Recombinant human interferon (IFN)-γ and TNF-α were obtained from PeproTech, Inc.
Imai Y., Chiba T., Kondo T., Kanzaki H., Kanayama K., Ao J., Kojima R., Kusakabe Y., Nakamura M., Saito T., Nakagawa R., Suzuki E., Nakamoto S., Muroyama R., Tawada A., Matsumura T., Nakagawa T., Kato J., Kotani A., Matsubara H, & Kato N. (2020). Interferon-γ induced PD-L1 expression and soluble PD-L1 production in gastric cancer. Oncology Letters, 20(3), 2161-2168.
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