Pmd19 t sample vector

Streptomyces griseus ATCC 10137^TM which produced the SGT was purchased from the American Type Culture Collection (ATCC, USA). P. pastoris GS115 (His⁻) and plasmid pPIC9K were both purchased from the Invitrogen^TM (Beijing, China). The pMD19-T Sample vector was purchased from TAKARA (Dalian, China). Yeast nutrient medium, minimal dextrose medium, minimal medium, yeast extract peptone dextrose medium, buffered minimal glycerol-complex medium and buffered minimal methanol-complex medium were prepared by means of “P. pastoris expression Kit” (Pichia Multi-Copy Expression Kit, version A, Invitrogen BV, The Netherlands.).

A. niger ND-1 (GenBank Accession number MH137707) was selected from samples collected in Chifeng, Inner Mongolia, China, and maintained in our laboratory. E. coli DH5α, P. pastoris strain X-33 and plasmid pPICZαA were purchased from Invitrogen (Beijing, China). All-in-One First-Strand Synthesis MasterMix with DNase I was from NOVA Yugong Biolabs (Jiangsu, China). pMD19-T sample vector, restriction enzymes, and ligases were from Takara. P-nitrophenyl (pNP) -β-D-xylopyranoside, p-nitrophenyl α-L-arabinofuranoside (pNP-α-L-Araf), pNPG, pNPC, CMC-Na, RBB-xylan, commercial endo-1,4-β-xylanase (XYL) from Thermomyces lanuginosus and ACR from Trichoderma reesei (ATCC 26921) were purchased from Sigma-Aldrich (United States). The extraction of total genomic DNA was performed by using DNeasy Blood & Tissue Kit (QIAGEN, Germany) as per manufacturer’s protocol. All chemicals were of analytical grade and commercially available.