All cell lines were obtained from the American Type Culture Collection (ATCC). MCF-7 and MDA-MB-468 (ATCC catalog number: HTB-22 and HTB-132 respectively) breast cancer cell lines were maintained in a 1:1 mixture of DME: F12 medium supplemented with 4 mM glutamine, 100 units/mL each of penicillin and streptomycin, and 10% heat-inactivated fetal bovine serum (FBS) and incubated at 37°C in 5% CO2. The MDA-MB-468 inducible expression cells were maintained in the same medium with addition of 50 µg/ml hygromycin and 0.2 µg/mL Puromycin. The HEK-293 cells (ATCC catalog number: CRL-1573) were maintained in DMEM medium with glutamine, antibiotics and 10% FBS. The cell lines resulted negative for mycoplasma contamination. AZD8055 was obtained from AstraZeneca Pharmaceuticals, rapamycin was purchased from EMD Bioscience. RAD001, KU006, WY354, PP242, MLN0128 were purchased from Tocris. Doxycycline was purchased from Sigma Aldrich. Puromycin and hygromycin stock solution were purchased from Invitrogen. Drugs were dissolved in DMSO to yield 10 mM stock and stored at −20°C.
Ku006
Manufactured by Bio-Techne
KU006 is a laboratory instrument designed to measure the concentration of specific molecules or proteins in a sample. It utilizes a technique called ELISA (Enzyme-Linked Immunosorbent Assay) to perform quantitative analysis. The core function of KU006 is to provide accurate and reliable measurement data for research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Puromycin, by Thermo Fisher Scientific (2 mentions)
Hygromycin, by Thermo Fisher Scientific (2 mentions)
Doxycycline, by Merck Group (2 mentions)
Wy354, by Bio-Techne (2 mentions)
Mln0128, by Bio-Techne (2 mentions)
Rad001, by Bio-Techne (2 mentions)
Pp242, by Bio-Techne (2 mentions)
Azd8055, by AstraZeneca (2 mentions)
2 protocols using ku006
1
Breast Cancer Cell Line Maintenance Protocol
2
Breast Cancer Cell Line Maintenance Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All cell lines were obtained from the American Type Culture Collection (ATCC). MCF-7 and MDA-MB-468 (ATCC catalog number: HTB-22 and HTB-132 respectively) breast cancer cell lines were maintained in a 1:1 mixture of DME: F12 medium supplemented with 4 mM glutamine, 100 units/mL each of penicillin and streptomycin, and 10% heat-inactivated fetal bovine serum (FBS) and incubated at 37°C in 5% CO2. The MDA-MB-468 inducible expression cells were maintained in the same medium with addition of 50 µg/ml hygromycin and 0.2 µg/mL Puromycin. The HEK-293 cells (ATCC catalog number: CRL-1573) were maintained in DMEM medium with glutamine, antibiotics and 10% FBS. The cell lines resulted negative for mycoplasma contamination. AZD8055 was obtained from AstraZeneca Pharmaceuticals, rapamycin was purchased from EMD Bioscience. RAD001, KU006, WY354, PP242, MLN0128 were purchased from Tocris. Doxycycline was purchased from Sigma Aldrich. Puromycin and hygromycin stock solution were purchased from Invitrogen. Drugs were dissolved in DMSO to yield 10 mM stock and stored at −20°C.
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