Anti mouse pe secondary antibody

To evaluate the properties of htMSCs differentiation, adherent cells underwent in vitro adipogenic, chondrogenic, and osteogenic differentiation using Life Technologies Stem Prodifferentiation medium kits (A1007101, A1007001, and A1007201), as indicated by the manufacturer. Flow cytometric analysis was provided for antihuman antibodies CD14 (VMRD Inc., Pullman, WA), CD29-PE-Cy5, CD31-PE, CD44-FITC, CD45-FITC, CD73-PE, CD90-R-PE, human leukocyte antigens- (HLA-) ABCFITC and HLA-DR-R-PE (Becton Dickinson), and SH4 (kindly provided by Dr. Irina Kerkis, Butantan Institute, São Paulo, Brazil). Unconjugated markers were reacted with anti-mouse PE secondary antibody (Guava Technologies). All methods were described before [6 (link), 7 (link)].

To analyze the cell-surface expression of typical protein markers, adherent cells were incubated with the following anti-human primary antibodies: CD31-phycoerythrin (PE), CD45-fluorescein isothiocyanate (FITC), CD90-R-PE, HLA-DR-R-PE (Becton–Dickinson and Company, Franklin Lakes, NJ). Unconjugated markers were reacted with anti-mouse PE secondary antibody (Guava Technologies, Hayward, CA). A total of 10,000 labeled cells were analyzed using a Guava EasyCyte flow cytometer running Guava ExpressPlus software (Guava Technologies).