Endometrial normal cells (EM-E6/E7/TERT) and cancer cells (AN3CA, HEC1A, KLE, RL-95-2) were kindly provided by Dr. Paul Goodfellow (The Ohio State University). Hec50co and Ishikawa H were kindly gifted by Dr. Kimberly K. Leslie (University of Iowa).8 (link) HEC1B, EFE-184, MFE-280, MFE-296 were purchased from ATCC (Manassas VA) or the DSMZ German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). All cell lines were cultured in a 37°C incubator with 5% CO2, according to the suppliers’ instructions, and were passaged for no more than 6 months after receipt or thawing. Cell line authentication was performed by IDEXX (Westbrook ME), which utilizes short tandem repeat (STR) profiling. To assess the role of DNA methylation in miR-137 expression, endometrial cancer cells were treated with 5-aza-2′-deoxycytidine (DAC; 5 μmol/L) for 48 hours, with or without trichostatin A (TSA, 0.5 μmol/L) (Sigma-Aldrich, St. Louis MO) for 24 hours.
Mfe 280
Manufactured by American Type Culture Collection
Sourced in United States
The MFE-280 is a high-performance microplate fermentation system designed for culturing and monitoring microbial growth. It provides precise control over temperature, agitation, and aeration to enable efficient fermentation experiments in a multi-well format.
Automatically generated - may contain errors
Lab products found in correlation
Mfe 296, by American Type Culture Collection (3 mentions)
Trichostatin a tsa, by Merck Group (2 mentions)
Hec 1b, by American Type Culture Collection (2 mentions)
Efe 184, by American Type Culture Collection (2 mentions)
5 aza 2 deoxycytidine, by Merck Group (2 mentions)
Sodium pyruvate, by Corning (1 mentions)
Rl95 2, by American Type Culture Collection (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Corning (1 mentions)
Mycoalert mycoplasma detection kit, by Lonza (1 mentions)
3 protocols using mfe 280
1
Endometrial Cancer Cell Lines Profiling
2
Endometrial Cancer Cell Lines Profiling
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Endometrial normal cells (EM-E6/E7/TERT) and cancer cells (AN3CA, HEC1A, KLE, RL-95-2) were kindly provided by Dr. Paul Goodfellow (The Ohio State University). Hec50co and Ishikawa H were kindly gifted by Dr. Kimberly K. Leslie (University of Iowa).8 (link) HEC1B, EFE-184, MFE-280, MFE-296 were purchased from ATCC (Manassas VA) or the DSMZ German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). All cell lines were cultured in a 37°C incubator with 5% CO2, according to the suppliers’ instructions, and were passaged for no more than 6 months after receipt or thawing. Cell line authentication was performed by IDEXX (Westbrook ME), which utilizes short tandem repeat (STR) profiling. To assess the role of DNA methylation in miR-137 expression, endometrial cancer cells were treated with 5-aza-2′-deoxycytidine (DAC; 5 μmol/L) for 48 hours, with or without trichostatin A (TSA, 0.5 μmol/L) (Sigma-Aldrich, St. Louis MO) for 24 hours.
3
Endometrial Carcinoma Cell Line Protocol
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Human endometrial carcinoma cell lines ECC1, HEC-1A, HEC-1B, HEC-50, HEC-59, Ishikawa, MFE-296, MFE-280 and RL-952 (ATCC) were cultured in RPMI-1640 with 2 nM L-glutamine (Invitrogen, Carlsbad, CA, USA) and 10% fetal bovine serum. Human USC cell lines ARK1 and ARK2 were a kind gift from Dr. Alessandro D. Santin (Yale Cancer Center, New Haven, CT, USA), and USC cell lines ACI-126 and ACI-158 were a kind gift from Dr. John I. Risinger (Michigan State University, East Lansing, MI, USA). All four cell lines were cultured in DMEM with 1.0 g/L glucose, L-glutamine, sodium pyruvate (Corning Cellgro, Corning, NY, USA) and 10% fetal bovine serum. All cells were maintained at 37 °C in 5% CO2. Use of the cell lines in experiments were within the first 10 passages. All cells were tested negative for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Group Ltd., Basel, Switzerland) at the time of thawing the frozen cell lines and were authenticated by short tandem repeat profiling in the Characterized Cell Line Core at the University of Texas MD Anderson Cancer Center.
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