Cryo-TEM analysis on selected samples was performed using a FEI CryoTitan (Thermo Fisher Scientific, Hillsboro, OR, USA) equipped with a field emission gun and autoloader and operated at 300 kV acceleration voltage in low-dose bright-field TEM mode. Samples for cryo-TEM were prepared by glow-discharging the grids (Lacey carbon coated, R2/2, Cu, 200 mesh, EM sciences) in a Cressington 208 carbon coater for 40 s. Then, 4 μL of the nanoparticle dispersion was pipetted onto the grid and blotted in a Vitrobot MARK III at room temperature and 100% humidity. The grid was blotted for 3 s (offset-3) and subsequently frozen in liquid ethane. Cryo-TEM images were acquired with zero loss energy filtering mode (Gatan GIF 2002, 20 eV energy slit) on a charge-coupled device (CCD) camera (Gatan model 794).
Mark 3
Manufactured by Cressington
The MARK III is a versatile laboratory instrument designed for general research and testing applications. It is capable of performing a range of analytical tasks. The product specifications and technical details are available upon request.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mark 3
1
Cryo-TEM Analysis of Nanoparticle Samples
2
Characterization of Nanoparticle Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cryo-TEM
experiments were conducted using a CryoTitan system (Thermo Fisher
Scientific) equipped with a field emission gun and autoloader, operating
at a 300 kV acceleration voltage in low-dose bright-field TEM mode.
To prepare samples for cryo-TEM, grids (Lacey carbon coated, R2/2,
Cu, 200 mesh, EM sciences) were glow-discharged in a Cressington 208
carbon coater for 40 s. Subsequently, 4 μL of the samples was
pipetted onto the grid and blotting was performed in a Vitrobot MARK
III at room temperature and 100% humidity. The grid was blotted for
3 s (offset −3) and then directly plunged and frozen in liquid
ethane. Cryo-TEM images were acquired in zero-loss energy filtering
mode (Gatan GIF 2002, 20 eV energy slit) using a CCD camera (Gatan
model 794). Dynamic light scattering (DLS) experiments were carried
out on a Malvern Z90 Zetasizer instrument equipped with a 633 nm He–Ne
laser. An avalanche photodiode detector was utilized to characterize
the hydrodynamic size of the particles, and scattering light at a
173° angle was detected for size and distribution analysis. The
pH change of the solution was monitored using a Mettler Toledo FiveEasy
Plus FEP20 pH Meter. Confocal laser scanning microscopy (CLSM) imaging
of cell samples was performed with a Leica TCS SP8X.
experiments were conducted using a CryoTitan system (Thermo Fisher
Scientific) equipped with a field emission gun and autoloader, operating
at a 300 kV acceleration voltage in low-dose bright-field TEM mode.
To prepare samples for cryo-TEM, grids (Lacey carbon coated, R2/2,
Cu, 200 mesh, EM sciences) were glow-discharged in a Cressington 208
carbon coater for 40 s. Subsequently, 4 μL of the samples was
pipetted onto the grid and blotting was performed in a Vitrobot MARK
III at room temperature and 100% humidity. The grid was blotted for
3 s (offset −3) and then directly plunged and frozen in liquid
ethane. Cryo-TEM images were acquired in zero-loss energy filtering
mode (Gatan GIF 2002, 20 eV energy slit) using a CCD camera (Gatan
model 794). Dynamic light scattering (DLS) experiments were carried
out on a Malvern Z90 Zetasizer instrument equipped with a 633 nm He–Ne
laser. An avalanche photodiode detector was utilized to characterize
the hydrodynamic size of the particles, and scattering light at a
173° angle was detected for size and distribution analysis. The
pH change of the solution was monitored using a Mettler Toledo FiveEasy
Plus FEP20 pH Meter. Confocal laser scanning microscopy (CLSM) imaging
of cell samples was performed with a Leica TCS SP8X.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!