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Apotrack cytochrome c apoptosis wb antibody cocktail

Manufactured by Abcam
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The ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail is a set of antibodies designed for the detection of proteins involved in the apoptosis pathway. The cocktail includes antibodies specific to cytochrome c and other key apoptosis-related proteins, enabling researchers to monitor the apoptosis process in their samples.

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Lab products found in correlation

Cell fractionation kit standard, by Abcam (1 mentions) Anti tom20, by Santa Cruz Biotechnology (1 mentions) Anti actin, by Merck Group (1 mentions) Mouse anti parkin, by Cell Signaling Technology (1 mentions) Abs1513 1, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions)

Close spelling variants of this product

Cytochrome C Antibody Cytochrome c

2 protocols using apotrack cytochrome c apoptosis wb antibody cocktail

1

Subcellular Fractionation of Cells Treated with Osimertinib and Trametinib

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Cells (15 × 104 cells / cm2) growing on 15 cm plates were treated with the combination of 100 nM osimertinib and 30 nM trametinib for 24 hours, and fractionated into mitochondrial and cytosolic fractions using Cell Fractionation Kit – Standard (ab109719, Abcam) according to the manufacturer’s instructions. Cytochrome c and ATP synthase subunit alpha (mitochondrial marker) was detected from the fractions by immunoblotting using ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415, Abcam). MEK 1/2 was used as the cytosolic marker (Cell Signaling #9122).
Kurppa K.J., Liu Y., To C., Zhang T., Fan M., Vajdi A., Knelson E.H., Xie Y., Lim K., Cejas P., Portell A., Lizotte P.H., Ficarro S.B., Li S., Chen T., Haikala H.M., Wang H., Bahcall M., Gao Y., Shalhout S., Boettcher S., Shin B.H., Thai T., Wilkens M.K., Tillgren M.L., Mushajiang M., Xu M., Choi J., Bertram A.A., Ebert B.L., Beroukhim R., Bandopadhayay P., Awad M.M., Gokhale P.C., Kirschmeier P.T., Marto J.A., Camargo F.D., Haq R., Paweletz C.P., Wong K.K., Barbie D.A., Long H.W., Gray N.S, & Jänne P.A. (2020). Treatment-induced tumor dormancy through YAP-mediated transcriptional reprogramming of the apoptotic pathway. Cancer cell, 37(1), 104-122.e12.
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2

Comprehensive Antibody Panel for Western Blotting

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Primary antibodies for western blotting were as follows: mouse anti‐Mfn1 (Abcam ab57602, 1:500), mouse anti‐Mfn2 (NeuroMab 75‐173, 1:500), mouse anti‐Mfn2 (Abcam ab56889, 1:500), mouse anti‐Parkin (Cell Signaling Technology 4211, 1:500), mouse anti‐PINK1 mouse (NeuroMab, Clone N357/6, 1:3), mouse anti‐VDAC1 (NeuroMab 75‐204 1:1,000), mouse anti‐ATP5a (from OXPHOS rodent antibody cocktail, Abcam ab110413, 1:2,000), rabbit anti‐Actin (Sigma A2066 1:1000), rabbit anti‐eIF2α (phospho S51) (Abcam ab32157, 1:500), rabbit anti‐eIF2α (Cell Signaling Technology 9722, 1:500) and mouse anti‐Ubiquitin (Enzo life sciences P4D1, 1:1,000). Primary antibodies for immunofluorescence were as follows: rat anti‐GFP (Nacalai Tesque 04404‐26, 1:2,000) and rabbit Anti‐phospho‐Ubiquitin (Ser65) (Merck Millipore ABS1513‐I, 1:300), ApoTrack™ Cytochrome C Apoptosis WB Antibody Cocktail (CValpha, PDH E1alpha, cytochrome C, GAPDH) was from Abcam (1:1,000, mouse), anti‐Actin (1:2,000, rabbit) and anti‐Flag (1:1,000, rabbit) were from Sigma, and anti‐Tom20 (1:500, rabbit) was from Santa Cruz Biotechnology. Anti‐myc (9E10) and anti‐HA were obtained from 9E10 and 12CA5 hybridoma lines, respectively, and used as supernatant at 1:100.
López‐Doménech G., Howden J.H., Covill‐Cooke C., Morfill C., Patel J.V., Bürli R., Crowther D., Birsa N., Brandon N.J, & Kittler J.T. (2021). Loss of neuronal Miro1 disrupts mitophagy and induces hyperactivation of the integrated stress response. The EMBO Journal, 40(14), e100715.
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