Calcium chloride (CaCl2), trans-4-(Aminomethyl) cyclohexanecarboxylicacid (AMCHA), dimethyl sulfoxide (DMSO), Ficoll-Paque density gradient medium, DAPI, and glutaraldehyde, were purchased from Sigma-Aldrich (Saint Louis, MO). Type I collagenase and Image-iTTM Green Hypoxia detection reagent and Triton X-100 were purchased from Thermo Fischer Scientific (Waltham, MA). Cell tracker DiO (excitation, 488 nm; emission, 525/50 nm) was purchased from Invitrogen (Carlsbad, CA). Drugs including PX-478 and Durvalumab were purchased from Selleck Chemicals (Houston, TX).
Ficoll paque density gradient medium
Manufactured by Merck Group
Sourced in United States
Ficoll-Paque density gradient medium is a laboratory product designed for the separation and purification of cells, organelles, and other biological particles. It is a sterile, non-toxic solution that utilizes a density gradient to facilitate the isolation of specific cell types or cellular components from complex mixtures.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Thermo Fisher Scientific (1 mentions)
Collagenase type 1, by Thermo Fisher Scientific (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Durvalumab, by Selleck Chemicals (1 mentions)
Px 478, by Selleck Chemicals (1 mentions)
D2650, by Merck Group (1 mentions)
2 protocols using ficoll paque density gradient medium
1
Hypoxia Detection and Cell Culture
2
Plasma Isolation and PBMC Cryopreservation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole blood was spun at 200×g for 10 min to separate plasma from cells. Plasma was then centrifuged at 1000×g for 10 min to remove platelets and the supernatant was stored at −80 °C till needed for antibody assays. PBMCs were isolated on a Ficoll-Paque density gradient medium (Histopaque-1077, Sigma), washed with 1X PBS, and resuspended in 90 % FBS/10 % DMSO (D2650, Sigma Aldrich USA). Cells were stored in liquid nitrogen in aliquots of 5–10 million per vial and thawed as needed for experiments.
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