Actin antibody a1978

P-TSC2 S939 (LS-C358381) was from Biozol (Sontheim, Germany). Antibodies specific for TSC2/tuberin (#4308), P-AKT S473 (#4060), AKT (#4691), P-S6K T389 (#9234), P-S S235/6 (#2211), S6 (#2217), P-ERK T202/Y204 (#4370), ERK (#4696), P-4E-BP T37/46 (#2855), as well as MG132 and cyclohexamide were from Cell Signaling (Danvers, MA, USA). Involucrin (SY8; ab20202) was from Abcam (Cambridge, UK), Actin antibody (A1978) was from Sigma-Aldrich (St. Louis, MO, USA) and LAMP-2 antibody (sc-18822) from SantaCruz Biotechnology (Dallas, TX, USA). LY294002, U0126 were from were from Calbiochem (San Diego, CA, USA).

All chemicals were purchased from Sigma unless stated otherwise. Rapamycin, Wortmannin, LY294002 and MHY1485 were from Calbiochem. Torin was purchased from Tocris Bioscience. Cytokines were obtained from Peprotech.
Phospho-specific (P-mTOR S2448 #5536, P-mTOR S2448 #2976, P-PRAS T246 #2997, P-S6 S235/6 #2211, P-Akt S473 #4060, P-ERk1 T202/Y204 #4370, P-p38MAPK T180/Y182 #4511, and corresponding pan antibodies (PRAS #2691, Akt #2938, S6 #2217) Rheb antibody (AP53656PU-N) was from Acris and tubulin #2128 antibody was from Cell Signaling Technology. Raptor antibody (sc27744) was from Santa Cruz, actin antibody (A1978) was from Sigma, involucrin antibody (ab20202) and β1-integrin antibody (ab30394) were from Abcam and filaggrin (PRB-417P-100) was from Convance. Ki-67 (MB67) antibody was obtained from Novus Biologicals and Keratin6 antibody (Ks6.KA12) from Thermo Scientific. Involucrin (Poly19244) for mouse IHC was from Biolegend.

FBS (F0926), thiazolyl blue tetrazolium bromide (MTT) (M2128), cisplatin (232120), PVDF membranes (IPVH00010), and actin antibody (A1978) were purchased from Sigma-Aldrich (St. Louis. MO, USA). Trypsin-EDTA (25300–054), DMEM (11995-065), bovine serum albumin (BP1605-100), Col17A1 (MA5-24848), goat anti-mouse Alexa fluor 680 IgG (A21058), goat anti-rabbit Alexa fluor 680 IgG (A21109), and Lipofectamine 2000 (11-668-019) were purchased from ThermoFisher Scientific (Waltham, MA, USA). Penicillin-streptomycin (SV30010) was purchased from GE Healthcare (Chicago, IL, USA). The PARP inhibitor olaparib (O-9201) was obtained from LC Laboratories (Woburn, MA, USA). The mTOR inhibitor rapamycin (13346), everolimus (11597), and chloroquine (30708) were obtained from Cayman Chemicals (Ann Arbor, MI, USA). The protein assay dye (500-0006) was purchased from Bio-Rad (Hercules, CA, USA). RIPA Buffer (9806), S100A9 (72590), p62 (5114), GAPDH (5174), and LC3 (2775) antibodies were purchased from Cell Signaling Technologies (Danvers, MA, USA).