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Eagle 2k camera

Manufactured by Thermo Fisher Scientific

The Eagle 2K camera is a high-performance digital camera designed for scientific and industrial applications. It features a 2048 x 2048 pixel CMOS sensor with a pixel size of 7.4 microns. The camera is capable of capturing images at a maximum frame rate of 30 frames per second. It provides a flexible interface, supporting both USB 3.0 and GigE connections.

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2 protocols using eagle 2k camera

1

Silver Nanoparticle Uptake Imaging

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The cells were incubated with silver nanoparticles for 3 hours and the samples for TEM were prepared according to the procedure reported previously.40 Serial sections of the fixed sample was cut and mounted on copper grids. A Philips EM 410 transmission electron microscope (FEI) was used to capture images with a FEI Eagle 2K camera.
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2

TEM Preparation of Human LEC Cultured on Hydrogels

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Human LECs cultured on hydrogels for 6 and 12 hours were prepared for TEM samples as previously described.25 (link) Briefly, cells were fixed with 3.0% formaldehyde, 1.5% glutaraldehyde in 0.1 M Na cacodylate, 5mM Ca2+ and 2.5% sucrose at room temperature for 1 hr and washed three times in 0.1 M cacodylate/2.5% sucrose pH 7.4 for 15 minutes each. The cells were post-fixed with Palade’s OsO4 on ice for 1 hour, rinsed with Kellenberger’s uranyl acetate and then processed conventionally through Epon embedding on a 16-well Lab-Tek chamber slide (NUNC). Serial sections were cut, mounted onto copper grids, and viewed using a Phillips EM 410 transmission electron microscope (FEI, Hillsboro, OR). Images were captured with a FEI Eagle 2k camera.
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