Zetaplus system

Manufactured by Brookhaven Instruments

The ZetaPlus system is a versatile instrument designed for the measurement of zeta potential and particle size distribution. It utilizes electrophoretic light scattering technology to determine the surface charge characteristics and size of particles suspended in a liquid medium.

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Lab products found in correlation

Zetasizer nano zs90, by Malvern Panalytical (1 mentions) Dls analyzer, by Brookhaven Instruments (1 mentions) Nir extended range pmt, by Horiba (1 mentions) Uv 2600, by Shimadzu (1 mentions) Fluoromax 4 fluorescence spectrometer, by Horiba (1 mentions)

2 protocols using zetaplus system

Evaluating Nano-ORL Formulations

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5 wt %, 10 wt %, and 20 wt % Nano-ORL formulations were synthesized in order to test the effect of ORL loading on NP size and zeta-potential. 0 wt % (empty NPs), 5 wt %, 10 wt %, and 20 wt % ORL formulations were dissolved in pure H₂O to a concentration of 1.0 mg/mL. Hydrodynamic diameter was then determined using a ZetaPlus system with onboard dynamic light scattering (DLS) analyzer (Brookhaven Instruments Corporation; Holtsville, NY). Zeta potential was determined using a Zetasizer Nano ZS90 (Malvern Instruments; Worcestershire, U.K.).

Hill T.K., Davis A.L., Wheeler F.B., Kelkar S.S., Freund E.C., Lowther W.T., Kridel S.J, & Mohs A.M. (2016). Development of a Self-Assembled Nanoparticle Formulation of Orlistat, Nano-ORL, with Increased Cytotoxicity against Human Tumor Cell Lines. Molecular pharmaceutics, 13(3), 720-728.

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Characterization of ICG-Loaded Nanoparticles

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NanoICG or empty NPs (empty NPs underwent the same dialysis treatment, but without ICG) were diluted to a concentration of 0.1 mg/mL in PBS and filtered through a 0.45 um filter (Fisher Scientific; Pittsburgh,PA). The hydrodynamic diameter (HD) of the NP-containing solutions was determined on ZetaPlus system with an onboard dynamic light scattering (DLS) analyzer (Brookhaven Instruments Corporation; Holtsville, NY). Absorbance (extinction) spectra were obtained on a UV-2600 (Shimadzu Scientific Instruments; Columbia MD). Fluorescence spectra were obtained on a FluoroMax-4 fluorescence spectrometer equipped with a NIR extended range PMT (Horiba Jobin Yvon; Edison, NJ). Spectra were obtained on aqueous solutions containing NPs and disassembled NP contents by addition of an equal volume of DMSO. ICG-loading content was determined with absorbance spectroscopy with a standard curve of ICG in 1:1 H₂O/DMSO.

Hill T.K., Abdulahad A., Kelkar S.S., Marini F.C., Long T.E., Provenzale J.M, & Mohs A.M. (2015). Indocyanine Green-Loaded Nanoparticles for Image-Guided Tumor Surgery. Bioconjugate chemistry, 26(2), 294-303.

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