Chrysin (>98% purity) was obtained from Yuanye (Shanghai, China). Chrysin was dissolved in dimethylsulfoxide (<1‰ DMSO) and freshly diluted in culture media for all in vitro experiments. Monoiodoacetic acid, lipopolysaccharide (LPS), type I collagenase and DMSO were all obtained from Sigma-Aldrich (Sigma, St.Louis, MO, USA). TRIzol, Dulbecco’s Modified Eagle Culture Medium (DMEM), Penicillin-Streptomycin mixture, fetal bovine serum (FBS) were purchased from Gibco (Rockville, USA). The primary antibodies for GAPDH, NLRP3, Caspase-1, Interleukin-18, Interleukin-1β and ASC were all purchased from Abcam (Cambridge, UK). Antibodies for c-Fos were purchased from Servicebio (Wuhan, China). Picro Sirius Red Stain kit and Goat anti-rabbit IgG H&L (HRP) were also supplied by Abcam (Cambridge, UK). In addition, 5×HiScript II qRt SuperMix and 2×ChamQ SYBR qPCR MsterMix (Low ROX Premixed) were obtained from Vazyme (Nanjing, China). The primers were supplied by Sangon Biotech (Shanghai, China). Caspase-1 Activity Assay Kit and Cell-Counting Kit-8 were obtained from Solarbio (Beijing, China). Enzyme-linked immunosorbent assays (ELISA) kits for Interleukin-1β and Interleukin-18 were supplied by Invitrogen (Life Technologies Corp., California, USA). ELISA kits for CGRP and SP were supplied by Jin Yibai (Nanjing, China).
Chrysin
Manufactured by Yuanye Bio-Technology
Sourced in China
Chrysin is a laboratory product manufactured by Yuanye Bio-Technology. It is a chemical compound used for research and analysis purposes in scientific settings.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin mixture, by Thermo Fisher Scientific (2 mentions)
Goat anti rabbit igg h l hrp, by Abcam (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Picro sirius red stain kit, by Abcam (1 mentions)
Dulbecco s modified eagle culture medium dmem, by Thermo Fisher Scientific (1 mentions)
Hiscript 2 q rt supermix, by Vazyme (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Gapdh, by Abcam (1 mentions)
Nlrp3, by Abcam (1 mentions)
Cell counting kit 8 (cck8), by Solarbio (1 mentions)
Caspase 1, by Abcam (1 mentions)
Caspase 1 activity assay kit, by Solarbio (1 mentions)
Baicalein, by Yuanye Bio-Technology (1 mentions)
Wogonin, by Yuanye Bio-Technology (1 mentions)
Nobiletin, by Merck Group (1 mentions)
Neohesperidin, by Merck Group (1 mentions)
Naringin, by Yuanye Bio-Technology (1 mentions)
Neoeriocitrin, by Yuanye Bio-Technology (1 mentions)
5 protocols using chrysin
1
Chrysin Modulates Inflammatory Pathways
2
Flavonoid Compound Procurement Protocol
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Naringenin, eriodictyol, apigenin, luteolin, liquiritigenin, butin, sakuranetin, kaempferol, quercetin, dihydrokaempferol, dihydroquercetin, eriocitrin, pinocembrin, narirutin, naringin, chrysin, genkwanin, baicalein, scutellarein, norwogonin, wogonin, and neoeriocitrin were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China). Hesperidin, neoHesperidin, sinensetin, isosinensetin, and nobiletin were obtained from Sigma-Aldrich (St Louis, MO, USA). 7-O-methyleriodictyol, isosakuranetin, and 5-HPMF were purchased from BioBioPha Co., Ltd (Kunming, China).
3
Chrysin Treatment of Gastric Cell Lines
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Human GC cell lines (SGC7901, MKN45 and BGC823) and the human gastric epithelial cell line GES-1 were grown in Dulbecco’s modified Eagle’s (DMEM; Gibco) supplemented with 10% fetal bovine serum (Gibco), and cultured at 37°C in 5% CO2. The human gastric epithelial cell GES1 served as control. Experiments were conducted by treating GES1, SGC7901, MKN45 and BGC823 cells with 40 μM of Chrysin (Yuanye Bio-Technology, Shanghai) for 48 h when they reached 80 to 90% confluence.
4
Chrysin Cytotoxicity in Cancer Cells
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Human TSCC cell lines SCC9 and CAL27, human gastric cancer cell line BGC823, and human hepatocellular carcinoma cell line HCC-LM3 were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco) or DMEM/F12, to which was added 10% fetal bovine serum (Gibco). SCC9 and CAL27 cells were treated with 40 μM of chrysin (Yuanye Bio-Technology, Shanghai) for 48 h.
5
Chrysin Treatment of Gastric Cell Lines
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The human gastric epithelial cell line GES-1 and human GC cell lines MKN-45 were obtained from Procell (Wuhan, China). Cells were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin mixture (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C in a humidified environment containing 5% CO2. 2 × 105 cells/mL cells at 80% confluence were cultured and pre-treated with Chrysin (Yuanye Bio-Technology, Shanghai) for 48 h.
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