The proteins from liver and cell extracts were subjected to Western blotting analysis with antibodies against FAS and SREBP-1. Protein expression levels were determined by an enhanced-chemiluminescence Western blotting kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) and LAS-4000 luminescent-image reader (Fuji Photo Film, Tokyo, Japan). The band intensities were analyzed using Fujifilm Image MultiGauge (Fuji Photo Film).
Las 4000 luminescent image reader
Manufactured by Fujifilm
Sourced in United States
The LAS-4000 is a luminescent-image reader designed for analyzing and quantifying luminescent signals. It is capable of capturing high-resolution images of luminescent samples, such as those used in Western blot, ELISA, and other luminescence-based assays. The LAS-4000 provides accurate and reproducible data for various applications in the life sciences and research fields.
Automatically generated - may contain errors
Lab products found in correlation
Image multigauge, by Fujifilm (3 mentions)
Anti p p38, by Cell Signaling Technology (2 mentions)
Anti nf κb p65 antibody, by Cell Signaling Technology (2 mentions)
Anti jnk and anti p38 antibodies, by Santa Cruz Biotechnology (2 mentions)
Chemiluminescent hrp substrate, by Merck Group (2 mentions)
Anti p nf κb p65, by Cell Signaling Technology (2 mentions)
Enhanced chemiluminescence western blotting kit, by Thermo Fisher Scientific (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti p jnk, by Cell Signaling Technology (1 mentions)
Anti p c jun n terminal kinase jnk, by Cell Signaling Technology (1 mentions)
3 protocols using las 4000 luminescent image reader
1
Western Blot Analysis of Liver Proteins
2
Protein Expression Analysis with Antibodies
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Anti-p-ERK, anti-ERK, anti-p-JNK, anti-p-p38, anti-p-NF-κB p65, and anti-NF-κB p65 antibodies (Cell Signaling Technology, Danvers, MA, USA) and anti-JNK and anti-p38 antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used to detect specific proteins. Protein expression was determined using a Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) and a LAS-4000 luminescent-image reader (Fuji Photo Film, Tokyo, Japan). Image MultiGauge (Fuji Photo Film) was used to analyze immunoreactive signals.
3
Quantifying MAPK and NF-κB Signaling
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For detection of MAPKs and NF-κB signaling pathways, anti-p- extracellular signal-regulated kinase (ERK), anti-ERK, anti-p-c-Jun N-terminal kinase (JNK), anti-p-p38, anti-p-NF-κB p65, and anti-NF-κB p65 antibodies (Cell Signaling Technology, Danvers, MA, USA) and anti-JNK and anti-p38 antibodies (Santa Cruz Biotechnology, Dallas, TX, USA) were used. Protein expression was determined using a Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) and a LAS-4000 luminescent-image reader (Fuji Photo Film, Tokyo, Japan). Image MultiGauge (Fuji Photo Film, Tokyo, Japan) was used to analyze immunoreactive signals.
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