The largest database of trusted experimental protocols

2 protocols using sulfachlorpyridazine

1

Multiresidue Analysis of Sulfonamides

Check if the same lab product or an alternative is used in the 5 most similar protocols
MeCN, methanol, n‐hexane, and formic acid were supplied by Merck. Ultrapure water was prepared with a Milli‐Q system by Millipore. All solvents were in chromatographic grade.
Analytical standards (≥98%) of sulfadiazine (SD), sulfadimethoxine (SDM), sulfamerazine (SM), sulfamethazine (SM2), sulfamethizole (SMT), sulfamethoxazole (SMZ), sulfathiazole (STZ) sulfachlorpyridazine (SCD), trimethoprim (TMP), sulfamethoxypyridazine (SMD), sulfapyridine (SPD), sulfadoxine (SDX), sulfisoxazole (SIZ), sulfabenzamide (SBA), sulfaquinoxaline (SQX), sulfacetamide (SAA), and sulfaphenazole (SPZ) were purchased from Sigma‐Aldrich. Stock solutions were prepared at a concentration of 100 µg/ml in methanol, and mixed standard solutions were diluted to 1,000 ng/ml. All solutions were stored at −20°C.
Oasis PRiME HLB (6cc, 200 mg) was purchased from Waters. EMR‐L product was purchased from Agilent Technologies, which consisted of two 15‐ml centrifuge tubes. QuEChERS d‐SPE EMR‐L contained 1 g EMR‐L material, and QuEChERS Final Polish EMR‐L contained 1.6 g magnesium sulfate and 0.4 g sodium chloride for salting out.
+ Open protocol
+ Expand
2

Quantification of Veterinary Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The reference standards sulfachlorpyridazine (SCP), doxycycline (DC) and enrofloxacin (ERF) were purchased at Sigma-Aldrich. Demeclocycline (DMC) was purchased at USP (Rockville, MD, United States), SCP-13 C 6 and ERF-d 5 were purchased at Witega (Berlin, Germany) and DC-d 3 at Toronto Research Chemicals (North York ON, Canada).
Stock solutions of SCP, DC and their internal standards SCP-13 C 6 , DC-d 3 and DMC were prepared in MeOH at a concentration of 1000 mg L -1 . ERF and ERF-d 5 were prepared in 5% ammonia/95% MeOH at a concentration of 100 mg L -1 . Mixed spike solutions of standards or internal standards were prepared in MeOH.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!