Ovarian cancer SKOV3 and A2780 cells were seeded (1×104) on the cover slip and allowed to adhere overnight. Twenty-four hours after transfection, cells were fixed, permeabilized and stained with Tetramethylrhodamine (TRITC)-conjugated phalloidin (Sigma-Aldrich, Louis, USA) for 2 hour. Nuclei were stained with Hochest (Sigma-Aldrich, Louis, USA) for 15 min. The results were analyzed with fluorescence microscope.
Hochest
Manufactured by Merck Group
Sourced in Germany
Hochest is a line of laboratory equipment produced by Merck Group. It is designed for general laboratory use, providing reliable and efficient performance for a variety of applications.
Automatically generated - may contain errors
Lab products found in correlation
Acumen ex3, by SPT Labtech (1 mentions)
Matrigel matrix, by Corning (1 mentions)
Anhydrous citric acid, by Merck Group (1 mentions)
N ethyl n 3 dimethylaminopropyl carbodiimide edc, by Merck Group (1 mentions)
Tsk g3000swxl, by Tosoh (1 mentions)
Sephadex g 15, by GE Healthcare (1 mentions)
Pd 10 desalting columns containing sephadex g 25 resin, by GE Healthcare (1 mentions)
Tsk g2000swxl, by Tosoh (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Dimethylformamide dmf, by Merck Group (1 mentions)
4 protocols using hochest
1
Ovarian Cancer Cell Cytoskeleton Imaging
2
Apoptosis Assessment via Hoechst-PI Staining
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Hoechst-PI staining assays was used to assess cell apoptosis. Cells were seeded in 96-well plates at a density of 5000 cells per well and incubated for 24 h. After washing with PBS, cells were stained with 0.2 mM/ml PI (Sigma-Aldrich, Germany) and 10 μg/ml Hochest (bisBenzimide H33342, Sigma-Aldrich, Germany) in PBS for 20 min at 37 °C in the dark. The Acumen® eX3 (TTP LabTech, Melbourne, UK) was used to scan and analyze the cells.
3
Matrigel Invasion Assay for Cancer Cells
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100 µl Matrigel matrix (Corning, USA) diluted in a 30:1 ratio with serum-free DMEM medium was added to the transwell chambers. 600 µl DMEM medium containing 10% FBS was added to the lower chamber and 5 × 105 cells were plated in the upper chamber. After incubation at 37°C for 120 h (HCCLM3) or 96 h (Hep3B), the invaded cells were fixed with 4% paraformaldehyde and stained with Hochest (bisBenzimide H33342, Sigma-Aldrich, Germany) for 15 min in the dark.
4
Carbodiimide-Mediated Synthesis of PEGylated Biomaterials
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Dicyclohexyl carbodiimide (DCC) (Cat # 802954), Dimethylformamide (DMF) (Cat # 103053), Poly (ethylene glycol) diacid (Cat # 843912), and anhydrous citric acid (Cat # 818707) were purchased from Merck. PD‐10 desalting columns containing Sephadex G‐25 resin (Cat # 170851) and Sephadex G15 (Cat # 170020) were purchased from GE Healthcare. N‐Hydroxysuccinimide (NHS) (Cat # 130672), RPMI 1640 (Cat # R8758), XTT (Cat # X4626), Hochest (Cat # B1155), and N‐Ethyl‐N′‐(3‐dimethylaminopropyl) carbodiimide (EDC) (Cat # E1769) were purchased from Sigma Aldrich. A549, L929, and NFS60 cell lines, rats, and mice were obtained from Iranian Genetic Resources Center and Pasteur Institute of Iran, respectively. G‐CSF was purchased from Cinnagen. Fetal bovine serum (FBS) (Cat # 10270) was purchased from GIBCO Invitrogen. Filter papers (Cat # DP 150‐150) and microplates (Cat # 3635) were purchased from Albet and corning, respectively. TSK G2000SWXL (Cat # 0008540), TSK G3000 SWXL (Cat # 0008541), and Guard (Cat # 0008543) were purchased from Tosoh. Quantikine ELISA kit for determination of G‐CSF (Cat # DCS50) was purchased from R&D systems.
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