Ab157592

For immunofluorescence staining, we used the following antibodies: mouse anti‐p34‐Arc (Dubois et al, 2005) (undiluted), mouse anti‐tubulin (Sigma T9026), rabbit anti‐pericentrin (ab4448), phalloidin‐FITC (P5282) and Alexa‐647 phalloidin (Fluka 65906). For inhibition experiments on isolated centrosomes, we used rabbit anti‐WASH antibodies (Derivery et al, 2009) (2 μg/ml) (gift Alexis).
For Western blot analysis, we used rabbit anti‐WASH1 (1: 1,000, ab157592, Abcam), rabbit anti‐WASH (1:1,000; Atlas), strumpellin (1: 1,000, ab101222, Abcam) and anti‐beta‐tubulin (sc‐5274, Santa Cruz). Labelled anti‐mouse and anti‐rabbit secondary antibodies (1: 1,000) and HRP‐conjugated goat IgG anti‐mouse and anti‐rabbit (1: 10,000) for Western blot were obtained from Jackson ImmunoResearch.
Cytochalasin D and nocodazole were purchased from Sigma‐Aldrich. PFA was purchased from Delta Microscopies. CK666 was purchased from Sigma‐Aldrich. Alexa‐647‐phalloidin was purchased from Life Technologies.

Cells were lysed with RIPA buffer (Solarbio, China) containing protease inhibitor cocktail (Sigma-Aldrich). Cell lysates were sonicated on ice, resolved on SDS-PAGE and transferred to a nitrocellulose membrane. After blocking with 5% non-fat milk, the membranes were probed with primary antibodies for the detection of WASH (ab157592, Abcam) and β-actin (3700S, Cell Signaling Technology) followed by horseradish peroxidase-conjugated secondary antibodies (Cell Signaling Technology). Protein expression was detected using enhanced chemiluminescent HRP substrate (Thermo Fisher Scientific) and photographed with a Chemiluminescence Imaging System (Bio-Rad Laboratories).