Image analysis system

Manufactured by Nikon

Sourced in Japan

The Image Analysis System is a specialized laboratory equipment designed for the purpose of processing and analyzing digital images. It provides tools and capabilities for tasks such as image segmentation, feature extraction, and quantitative measurements. The core function of this system is to facilitate the objective and precise examination of visual data in a variety of research and analytical applications.

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Lab products found in correlation

Millipore membrane filter, by Merck Group (2 mentions) Qcl 1000, by Lonza (2 mentions) Eclipse 80i light microscope, by Nikon (2 mentions) Ds ri1 digital camera, by Nikon (1 mentions) Eclipse te2000 u microscope, by Nikon (1 mentions) Shp125, by ScyTek Laboratories (1 mentions) Mayer s hematoxylin, by Bio-Optica (1 mentions) Bs 2081r, by Bioss Antibodies (1 mentions) Bs 0081r, by Bioss Antibodies (1 mentions)

Close spelling variants of this product

NIS-Elements BR 3.2 image analysis system (9 citations) H600L Microscope and image analysis system (3 citations) NIS elements BR 4.13.00 64-bit image analysis system (3 citations) Digital image analysis system (2 citations) LuciaG Image Analysis System (2 citations) NIS-Elements advanced research image acquisition and analysis system (2 citations)

5 protocols using image analysis system

Fluorescence Microscopy of DNA Molecules

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Brightfield and fluorescence
microscopy observations were performed
on an Eclipse TE 2000-U microscope (Nikon Instruments Inc.) equipped
with a blue excitation B-2A filter (Nikon Instruments Inc.) with longpass
emission (excitation 450–490 nm, cutoff wavelength 500 nm,
and barrier filter wavelength 515 nm) and oil-immersed ×100 or
×40 objective lens. YOYO-labeled T4 DNA molecules were illuminated
with a mercury lamp, and fluorescent images were observed and recorded
on a EB-CCD monochrome camera using an Argus 10 image processor system
(Hamamatsu Photonics, Japan). Fluorescence images of beads containing
DNA and multilayers and capsules were recorded on a Nikon DS-Ri1 digital
camera and analyzed using a Micron Optics image-analysis system and
NIS-Elements BR 3.1 software. The focal plane of beads’ and
capsules’ images was chosen at approximately the center of
the bead/capsule height.
The size of DNA molecules and beads
was measured using ImageJ 1.52i software (NIH). The apparent long-axis
length of the T4 DNA molecule in solution was measured as the longest
distance in the outline of DNA molecule fluorescence images of single-DNA
chain. The diameter of beads and capsules was measured at approximately
the center of bead/capsule height.

Zinchenko A., Inagaki E, & Murata S. (2019). Encapsulation of Long Genomic DNA into a Confinement of a Polyelectrolyte Microcapsule: A Single-Molecule Insight. ACS Omega, 4(1), 458-464.

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Preparation of Calibrated Titanium and Alumina Particles

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Commercially pure titanium particles of 4.507 g/cm³ were purchased from Alfa Aesar (Ward Hill, MA, USA), and alumina ceramic particles of 3.53 g/cm³ from Ceram Tec (Plochingen, Germany). All particles were disseminated in pure water and filtered through Millipore filter membranes (Billerica, MA, USA) of different diameters (0.2 μm and 1.2 μm) to select particles with a target size between 0.2 μm and 1.2 μm. An image analysis system (NIKON, Japan) was used to measure the exact size of the particles, and the results were 0.82 ± 0.12 μm and 0.84 ± 0.14 μm for the filtrated titanium and ceramic particles, respectively. All particles were rinsed with 70 % ethanol for 24 h at room temperature and then dried in an oven before being sterilized with ethylene oxide. Based on particle weight and density, particles incubated in phosphate buffered saline (PBS) were adjusted to 4 × 10⁸ μm³/ml in concentration. The level of endotoxin in particle solutions was detected using the Limulus Amebocyte Lysate Assay (QCL-1000; Bio Whittaker, Walkersville, Maryland, USA), and the result was below the detection level of 0.01 EU/ml.

Qin C.Q., Huang D.S., Zhang C., Song B., Huang J.B, & Ding Y. (2016). Lentivirus-mediated short hairpin RNA interference targeting TNF-alpha in macrophages inhibits particle-induced inflammation and osteolysis in vitro and in vivo. BMC Musculoskeletal Disorders, 17, 431.

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Titanium Particle Characterization and Purification

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Titanium particles were supplied by Alfa Aesar (Ward Hill, MA, USA). The titanium particles were deliquated with deionized water and filtered through Millipore filter membranes (Billerica, MA, USA) of a range of sizes (0.2, 1.2, and 10 μm). We harvestedthree different sizes of the particle after filtration, 0.2-1.2 μm (TI-0.2), 1.2-10 μm and >10 μm. The particles were washed in 70% ethanol for 24 hours to remove endotoxin and further sterilized by ethylene oxide. The particles were then suspended in PBS and modulated to 4 × 10 8 mm 3 /ml. The presence of endotoxin in the particle solutions was determined using limulus amebocyte lysate assay (QCl-1000; Bio Whittaker, Walkersville, MD, USA), which was shown lower than 0.01 EU/ml. The precise sizes of the particles were determined by the image analysis system (NIKON, Tokyo, Japan) , which found that there was no significant difference in size among the titanium particles.

Li Y., Lin S., Liu P., Huang J., Qiu J., Wen Z., Yuan J., Qiu H., Liu Y., Liu Q., Zhou T., Luo P., Guo H., Ma Y., Guo D., Mo G., Tang Y., Xu L., L.i.a.n.g., Xu J., Ding Y, & Zhang S. (2021). Carnosol suppresses RANKL-induced osteoclastogenesis and attenuates titanium particles-induced osteolysis. Journal of cellular physiology, 236(3).

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Immunohistochemical Analysis of TNFα and Caspase-3

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For immunohistochemical analysis, sections were mounted on polylysine coated slides. After rehydrating, samples were transferred to citrate buffer (pH 7.6) and heated in a microwave oven for 20 minutes. After cooling for 20 minutes at room temperature, the sections were washed with phosphate buffered saline (PBS). Then sections were kept in 0.3% H 2 O 2 for 7 minutes and afterward washed with PBS. Sections were incubated with at an anti-TNFα (1:100, bs-2081R, Bioss, China) and anti-Caspase 3 (1:100, bs-0081R, Bioss, China) for 60 minutes. They then were rinsed in PBS and incubated with biotinylated goat antipolyvalent for 10 minutes and streptavidin peroxidase (SHP 125, ScyTek Laboratories, ABD) for 10 minutes at room temperature. Staining was completed with chromogen + substrate for 15 minutes, and slides were counterstained with Mayer's hematoxylin (M06002, Bio-optica, ITALY) for 1 minute, rinsed in tap water, and dehydrated. The antibody was used according to the manufacturer's instructions. Staining for anti-TNFα and anti-caspase-3 was identi ed by a brown color. The relative intensity of TNFα and caspase-3 immunostaining was scored as follows: 0-5% (+), 6-20% (++), 21-40% (+++), 41-60% (++++), 61-80% (+++++), and 81-100% (++++++). All sections were examined using a Nikon Eclipse 80i light microscope and Nikon Image Analysis system.

Abdulkareem Aljumaily S.A., Demir M., Elbe H., Yigitturk G., Bicer Y, & Altinoz E. (2021). Antioxidant, anti-inflammatory, and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats. Environmental science and pollution research international, 28(46).

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Histological Evaluation of Cardiac Damage

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The heart tissues were xed in 10% neutral buffered formalin for 48h. Tissues were washed in running water, and were dehydrated with increasing concentrations of ethanol (50%, 75%, 96% and 100%). After dehydration, specimens were placed into xylene to obtain transparency and were embedded in para n. Para n blocks were cut at 5 µm, mounted on slides, stained with hematoxylin and eosin (H-E). The tissue sections were examined under light microscopy. The evaluated parameters for severity of cardiac damage were congestion, necrosis, in ltration and loss of myo brillar in 10 different elds for each section. For this analysis, cardiac damage was semiquantitatively graded as absent (0), mild (1), moderate (2), and severe (3), for each criterion. The maximum damage score was 12. All sections were examined using a Nikon Eclipse 80i light microscope and Nikon Image Analysis system.

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