Cells were stained in PBS containing 1% (w/v) bovine serum albumin on ice for 30 min, with anti-IgG1 (RMG1–1), anti-CD19 (ID3), anti-B220 (RA3–6B2), anti-CD4 (GK1.5), anti-CD8α (53–6.7), anti-CD25 (PC16), anti-GL7 (GL-7), anti-CD138 (281–2), anti-IgD (11–26c.2a), anti-CD95 (Jo2), anti-PD-1 (J43), anti-IgM (II/41), and anti-CXCR5 (2G8). Antigen specific GC response was detected with PE-conjugated anti-NP tetramer. Cell viability was assessed using the Fixable Dye Ghost 510. Phosflow staining was performed using Phosflow Fix/Perm kit (BD Biosciences). Flow cytometry was performed on the LSR II, LSR Fortessa (BD Biosciences) or Attune NxT (Thermo Fisher) cytometers, and analysis was performed using FlowJo software.
Phosflow fix perm kit
Manufactured by BD
The Phosflow Fix/Perm kit is a laboratory equipment product designed for the fixation and permeabilization of cells for the analysis of intracellular phosphoproteins. The kit provides the necessary reagents to prepare samples for flow cytometry analysis.
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Lab products found in correlation
2 protocols using phosflow fix perm kit
1
Multiparametric Immunophenotyping of B Cell Responses
2
Multiparametric Immunophenotyping of B Cell Responses
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Cells were stained in PBS containing 1% (w/v) bovine serum albumin on ice for 30 min, with anti-IgG1 (RMG1–1), anti-CD19 (ID3), anti-B220 (RA3–6B2), anti-CD4 (GK1.5), anti-CD8α (53–6.7), anti-CD25 (PC16), anti-GL7 (GL-7), anti-CD138 (281–2), anti-IgD (11–26c.2a), anti-CD95 (Jo2), anti-PD-1 (J43), anti-IgM (II/41), and anti-CXCR5 (2G8). Antigen specific GC response was detected with PE-conjugated anti-NP tetramer. Cell viability was assessed using the Fixable Dye Ghost 510. Phosflow staining was performed using Phosflow Fix/Perm kit (BD Biosciences). Flow cytometry was performed on the LSR II, LSR Fortessa (BD Biosciences) or Attune NxT (Thermo Fisher) cytometers, and analysis was performed using FlowJo software.
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