Promastigote forms were treated with OGSA at 100, 450, or 900 μg/mL for 24 h at 25°C and incubated with 1 μL (5 mg/mL in ethanol) rhodamine 123 (Rh 123; Sigma-Aldrich, St. Louis, MO, USA) for 15 min. Parasites treated with the protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP; Sigma-Aldrich; 100.0 μM) were used as a positive control. CCCP is a known mitochondrial uncoupler that allows proton transportation to the mitochondrial matrix, causing mitochondrial transmembrane potential depolarization [21 (link)]. Ten thousand events were analyzed using a BD FACSCalibur flow cytometer, and the data were analyzed on CellQuest Pro software [22 (link)]. Parasites exhibiting mitochondrial depolarization were represented in a histogram, using FL-2 as a parameter.
3 chlorophenylhydrazone
Manufactured by Merck Group
Sourced in United States, France
3-chlorophenylhydrazone is a chemical compound used as a lab reagent. It functions as a general-purpose indicator and can be used to detect the presence of certain chemical species. The compound has a specific molecular structure and physical properties that make it suitable for various analytical and experimental applications in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Rhodamine 123, by Merck Group (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Hek293 cell line, by American Type Culture Collection (1 mentions)
Celltiter 96 aqueous non radioactive cell proliferation assay mts, by Promega (1 mentions)
Charybdotoxin, by Alomone (1 mentions)
Fluo 4 am, by Thermo Fisher Scientific (1 mentions)
Gsmtx4, by Alomone (1 mentions)
3 protocols using 3 chlorophenylhydrazone
1
Mitochondrial Depolarization Assay for Leishmania
2
Cytotoxicity and Hepatotoxicity Evaluation
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Cytotoxicity and hepatotoxicity
were estimated using the human
embryonic kidney HEK-293 cell line (ATCC CRL-1573) and hepatoma HepG2
(ATCC HB-8065) cell line. The CellTiter 96 Aqueous Non-Radioactive
Cell Proliferation Assay (MTS) was purchased from Promega (Madison,
WI, USA) and performed according to the manufacturer’s protocol.
The viability of cells was assessed after 72 h of incubation with
PF9601N, ASS234, and contilisant in quadruplicate at four concentrations
(1, 10, 50, and 100 μM). The antiproliferative drug doxorubicin
(DX) (1 μM) and mitochondrial toxin carbonyl cyanide 3-chlorophenyl-hydrazone
(CCCP) (10 μM, only in hepatotoxicity assay) were used as positive
controls (purchased from Sigma-Aldrich, St. Louis, MO, USA).
were estimated using the human
embryonic kidney HEK-293 cell line (ATCC CRL-1573) and hepatoma HepG2
(ATCC HB-8065) cell line. The CellTiter 96 Aqueous Non-Radioactive
Cell Proliferation Assay (MTS) was purchased from Promega (Madison,
WI, USA) and performed according to the manufacturer’s protocol.
The viability of cells was assessed after 72 h of incubation with
PF9601N, ASS234, and contilisant in quadruplicate at four concentrations
(1, 10, 50, and 100 μM). The antiproliferative drug doxorubicin
(DX) (1 μM) and mitochondrial toxin carbonyl cyanide 3-chlorophenyl-hydrazone
(CCCP) (10 μM, only in hepatotoxicity assay) were used as positive
controls (purchased from Sigma-Aldrich, St. Louis, MO, USA).
3
Pharmacological Modulation of Ion Channels
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All salts were acquired from Sigma and were of analytical grade or better. Dooku1: 2-[(2,6-Dichlorobenzyl)thio)-5-(1H-pyrrol-2-yl)-1,3,4-oxadiazole (Sigma-Aldrich, France). Yoda1: 2- [5-[[(2,6-Dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]pyrazine (Tocris, France). Charybdotoxin (Alomone labs, Israel). CCCP: carbonyl cyanide 3-chlorophenylhydrazone (Sigma-Aldrich, France). GsMTx4 (Alomone labs, Israel). Fluo-4, AM (Invitrogen, France). All drugs are used at 1000X stock solution in DMSO, except Charybdotoxin and GsMTx4 in water.
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