Oxidized AAT was prepared from native plasma-purified AAT (see above) by adding N-chlorosuccinimide (Sigma-Aldrich, Merck, Darmstadt, Germany) to AAT solution at a molar ratio of 20:1. The mixture was allowed to react for 20 min at room temperature, and N-chlorosuccinimide was then removed by washing with PBS 3 times using viva spin-20 centrifugal columns with a 10 k MWCO (Sartorius, Göttingen, Germany).
N chlorosuccinimide
Manufactured by Sartorius
Sourced in Germany
N-chlorosuccinimide is a chemical reagent commonly used in organic synthesis. It is a chlorinating and oxidizing agent that can be utilized in various reactions. The core function of N-chlorosuccinimide is to introduce chlorine atoms or facilitate oxidation processes during the synthesis of organic compounds.
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Lab products found in correlation
2 protocols using n chlorosuccinimide
1
Oxidation of Plasma-Purified AAT
2
Oxidation of Alpha-1 Antitrypsin
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Oxidized AAT was prepared by adding N-chlorosuccinimide (Sigma, St. Louis, MO, USA) to native AAT dissolved in PBS at 25:1 molar ratio. The mixture was allowed to react for 30 min at RT, and N-chlorosuccinimide was then removed by washing and equilibrated with PBS using viva spin-20 centrifugal columns (Sartorius, Gottingen, Germany). Oxidized AAT did not form complex with elastase as it has been shown in our previous studies [29 (link)].
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