The active substance used was cetirizine dihydrochloride (Jubilant Lifesciences Ltd, India; CTZ). The excipients used for the study SeDeM were talc (Fagron, Spain), magnesium stearate (Fagron, Spain), and colloidal silicon dioxide (Aerosil ®, Fagron, Spain). The excipients used in the factorial design were mannitol (Fagron, Spain) and polyvinylpyrrolidone Ph. Eur (Fagron, Spain). The reagents used for the preparation of phosphate buffers solutions (solubility studies) were: 37% HCl (Panreac, Barcelona), NaCl (Panreac, Barcelona), NaOH (Panreac, Barcelona) and KH2PO4 (Panreac, BCN).
Mannitol
Manufactured by Fagron
Sourced in Spain, Germany, Belgium
Mannitol is a type of sugar alcohol that is commonly used in pharmaceutical and dietary applications. It is a crystalline solid that is water-soluble and has a sweet taste. Mannitol is primarily used as an excipient in various pharmaceutical formulations, where it serves as a diluent, sweetener, and bulking agent.
Automatically generated - may contain errors
Lab products found in correlation
Magnesium stearate, by Fagron (2 mentions)
Lactose, by Fagron (2 mentions)
Sodium hydroxide (naoh), by ITW Reagents (1 mentions)
Sodium chloride (nacl), by ITW Reagents (1 mentions)
Aerosil, by Fagron (1 mentions)
Kh2po4, by ITW Reagents (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
Ferric chloride, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (1 mentions)
2 4 6 tris 2 pyridyl s triazine tptz, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Nadph, by Merck Group (1 mentions)
Feso4 7h2o, by Merck Group (1 mentions)
Monosodium phosphate, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
4 protocols using mannitol
1
Cetirizine Formulation Development and Evaluation
2
Antioxidant Activity Assessment of Melatonin Formulation
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Melatonin was purchased from Methapharmaceutical IND S.L. (Spain). Mannitol, polyvinylpyrrolidone (crospovidone), lactose, magnesium stearate, anhydrous colloidal silica, tartaric acid and sodium bicarbonate were all supplied by Fagron Ibérica S.A.U. (Spain).
The antioxidants, vitamin C (sodium ascorbyl phosphate) and resveratrol were supplied by Fagron Ibérica S.A.U. (Spain). NADPH, adenosine triphosphate (ATP) and glutathione (GSH) were purchased from Sigma Aldrich (Spain).
For the determination of total antioxidant activity using the ferric ion reducing antioxidant power (FRAP) technique, the following were used: sodium acetate, acetic acid, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), hydrochloric acid, ferric chloride, FeSO4 × 7 H2O and bidistilled water, purchased from Sigma Aldrich (Spain).
For the determination of total antioxidant activity using the TAC technique, the following were used: sodium acetate, bidistilled water, glacial acetic acid, hydrogen peroxide, 2,2’-azino-bis-2-ethybenzothiazoline-6-sulphonic acid (ABTS), the water-soluble analogue of vitamin E (Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)), ethanol, methanol and monosodium phosphate, purchased from Sigma Aldrich (Spain).
The antioxidants, vitamin C (sodium ascorbyl phosphate) and resveratrol were supplied by Fagron Ibérica S.A.U. (Spain). NADPH, adenosine triphosphate (ATP) and glutathione (GSH) were purchased from Sigma Aldrich (Spain).
For the determination of total antioxidant activity using the ferric ion reducing antioxidant power (FRAP) technique, the following were used: sodium acetate, acetic acid, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), hydrochloric acid, ferric chloride, FeSO4 × 7 H2O and bidistilled water, purchased from Sigma Aldrich (Spain).
For the determination of total antioxidant activity using the TAC technique, the following were used: sodium acetate, bidistilled water, glacial acetic acid, hydrogen peroxide, 2,2’-azino-bis-2-ethybenzothiazoline-6-sulphonic acid (ABTS), the water-soluble analogue of vitamin E (Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)), ethanol, methanol and monosodium phosphate, purchased from Sigma Aldrich (Spain).
3
Formulation and Characterization of Contrast Agents
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PEG standards, polyvinyl alcohol (PVA 13-23) and sodium dodecyl sulphate (SDS) were purchased from Sigma Aldrich (Zwijndrecht, The Netherlands). Carboxymethyl cellulose (CMC) was purchased from Aqualon (Barendrecht, The Netherlands). Dichloromethane (DCM, p.a. stabilized with EtOH), sodium azide (NaN 3 ) and Tween-20 were purchased from Across (Geel, Belgium). Ultrapure water was purchased from B. Braun Medical B.V. (Oss, The Netherlands). Mannitol was purchased from Fagron (Barbsbütel, Germany). NIR780 was purchased from Li-Cor Inc (Nebraska, USA). Lumogen F Red 300 (Perylene red) was purchased from BASF (Ludwigshafen, Germany). Hexabrix 320 Ò , a clinical iodine-based contrast agent, was obtained from Guerget, The Netherlands. All reagents were used as received.
4
Freeze-Drying of Protein Formulations
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Five different formulations having a different specific dry product resistance were selected from literature (Kuu et al., 2006; Overcashier et al., 1999) (Table 1). Trehalose was purchased from Cargill (Germany). Polysorbate 20, sodium chloride, lactose and mannitol were purchased from Fagron (Belgium). L-histidine and glycine were purchased from Sigma-Aldrich (United States).
Prior to freeze-drying, 10ml type I glass vials were filled with a specific volume of the formulation (see section 4.2).
After Freezing (see section 4.1.), all frozen vials were dried in an Amsco FINN-AQUA GT4 freeze-dryer (GEA, Köln, Germany).
Prior to freeze-drying, 10ml type I glass vials were filled with a specific volume of the formulation (see section 4.2).
After Freezing (see section 4.1.), all frozen vials were dried in an Amsco FINN-AQUA GT4 freeze-dryer (GEA, Köln, Germany).
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