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2 protocols using resomer rg 756 s

1

Characterization of PLGA Polymers

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Three different PLGAs were purchased from Sigma-Aldrich (Steinheim, Germany): PLGA (1) Resomer® RG 752 H, 75:25, acid terminated, 4,000–15,000 Da; PLGA (2) PLGA, 85:15, ester terminated, 50,000–75,000 DA; PLGA (3) Resomer® RG 756 S, 75:25, ester terminated, 76,000–115,000 Da. Subsequently, PLGAs are named with PLGA 1, PLGA 2, and PLGA 3. Ethyl acetate from Sigma-Aldrich (Steinheim, Germany) was used for preparing PLGA solutions.
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2

Polymer-Based Drug Delivery Platform

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Poly (D,L,lactide-co-glycolide acid) (PLGA) Resomer® RG-504, (50:50, Mw 38000–54000), Resomer® RG 756 S (75:25, Mw 76,000–115,000), Sodium Dodecyl Sulphate (SDS), Acrylamide, methylene-bis-Acrylamide (MBA), dioctyl sulfosuccinate, Brij 30, hexane, ammonium persulfate (APS), and N,N,N′,N′-tetramethylethylenediamine (TEMED), were all acquired from Sigma-Aldrich (St. Louis, MO). N-(3-aminopropyl) methAcrylamide hydrochloride salt (APMA) was obtained from Polysciences Inc. (Warrington, PA). Ethyl acetate and Methanol were obtained from Fisher Chemical. Ethanol (95%) was acquired from Decon Labs, Inc. (King of Prussia, PA). The LysoTracker Red DNB-99, MitoTracker Green FM, and Singlet oxygen sensor green (SOSG) probes were obtained from Invitrogen (Thermo Scientific; Waltham, MA). 2-devinyl-2-(1-hexyloxyethyl) pyropheophorbide (HPPH) was obtained as a gift from the Department of Dermatology, Roswell Park Cancer Institute. All solutions were prepared in ACS reagent grade water. All the chemicals and materials were used as received.
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